100 glycan microarray service provided by CD BioGlyco helps researchers determine the binding partners for 100 diverse glycans in serum, proteins, plasma, cell cultures, cell/tissue lysates, or other potential biological samples, which represent the range of glycosylations found on cell membranes. We look forward to providing you with efficient scientific services in your research related to glycan microarray analysis.

Introduction

Glycocalyx is an extracellular polymer coating surrounding many eukaryotic and prokaryotic cells, consisting of glycolipids, glycoproteins, proteoglycans and glycosaminoglycans, which play important roles in cell signaling, viral infection and immune processes. Therefore, the clarification of glycan-associated biomolecular interactions and their consequences is critical for fundamental biology research and biomedical applications. However, there are currently very limited assay tools available for glycobiology research.

CD BioGlyco has worked to develop glycan microarrays for the rapid analysis of glycan-related recognition events. Because they contain numerous glycans immobilized in a compact and ordered manner on a solid surface, glycan microarrays allow for multiple parallel analyses of glycan-protein interaction using only a small number of glycan samples. Microarray technology is now being used as an effective tool to elucidate the role of glycans and glycan-binding proteins in biological systems.

Fig.1 Major classes of glycans in mammalian cells. (Hyun, 2017)

Our Services

CD BioGlyco provides customers with microarray analysis of 100 synthetic glycans. The most common identified structures displaying important binding functions in the literature are included, such as β-Glc-sp, β-Gal-sp, α-Man-sp, β-GlcNAc-sp, Neu5Ac-α-2,3-Gal-β-1,3-GlcNAc-β-sp, Gal-α-1,4-Gal-β-1,4-Glc-β-sp, GlcNAc-β-1,6-GlcNAc-β-sp, Neu5Gc-α-2,3-Gal-β-1,3-(Fuc-α-1,4)-GlcNAc-β-sp [Sialyl Lewis A], GlcNAc-β-1,6-(Gal-β-1,3)-GalNAc-α-O-Ser, GlcNAc-β-1,4-GlcNAc-β-sp, Gal-β-1,3-GalNAc-β-sp, Gal-α-1,3-(Fuc-α-1,2)-Gal-β-1,4-Glc-β-sp [Blood B antigen tetrasaccharide], GalNAc-β-1,4-(Neu5Ac-α-2,8-Neu5Ac-α-2,3)-Gal-β-1,4-Glc-β-sp [GD2], Neu5Ac-α-2,8-Neu5Ac-α-2,6-Gal-β-1,4-Glc-sp, etc. For more information about glycans, please contact us directly.

We provide 100 glycan microarray services to help researchers:

• Determination of glycans binding partners in various biological samples
• Rapid profiling of glycan-binding properties of proteins
• Evaluation of substrate specificities of several glycosyltransferases
• Screening for glycans that promote cell surface lectin-related cellular responses
• Study on inflammatory immune responses

Advantages of Us

• Scalable - our high throughput instruments provide microarray analysis of a wide range of glycans.
• Reliable - we have years of experience servicing the glycobiology field.
• Complete - complete process from sample preparation to the interpretation of the results.

Equipped with high throughput and automated facilities, CD BioGlyco has developed glycans array analysis platform for screening protein-carbohydrate interactions. Our high-efficient systems help customers reduce project costs and timelines to accelerate their research. If you need our scientific services, please contact us  for further consultation.

Reference:

1. Hyun, J.Y.; et al. The glycan microarray story from construction to applications. Acc Chem Res. 2017, 50(4):1069-1078.