100 N-glycan microarray service of CD BioGlyco helps researchers investigate the binding properties of proteins, antibodies, cell cultures, bacteria or other potential biological samples to an array of 100 basic N-glycans. The glycan microarray platform established by our company ensures that we provide customers with high-quality scientific services to accelerate their research.

Introduction

N-linked protein glycosylation is a commonly occurring co-translational and post-translational modification and it is estimated that the majority of all secreted proteins are N-glycosylated. N-glycosylation modifications play an important role in the proper folding, functional localization, and intracellular transport of proteins. In mammals, N-glycosylation is important for the regulation of embryonic development, cancer development, and immune defense. In plants, N-glycosylation is significant for many physiological processes such as cell wall formation, reproductive development and defense against pathogenic bacteria invasion.

Complex N-glycans are involved in a wide range of cellular processes in mammals, especially in intercellular interactions and immune cell functions, so they are generally considered by researchers as the preferred glycan candidates for the investigation of carbohydrate-binding-vaccines like HIV broadly neutralizing antibodies (bNAb). The basic N-glycan structures offered in our 100 N-glycan microarray could help researchers understand the binding determinants underlying antibodies or other proteins of interest.

Fig.1 Elucidation of glycan-binding proteins (GBP) specificity by defined glycan microarrays. (Arthur, 2014)

Our Services

CD BioGlyco provides customers with microarray service for 100 N-glycans, including 81 complex and hybrid N-glycans, 10 high-mannose N-glycans and 9 Neu5Gc-N-glycans. The service we provide include but are not limited to:

• Traditional glycan microarrays. This method requires very small amounts of test glycans to provide detailed information on the effect of glycan modification on GBP recognition.
• Shotgun glycan microarrays, a functional glycomics approach, provide for the characterization of the actual glycan ligands separated from natural sources for a specific GBP.
• Microbial glycan microarrays to examine the specificity of host factors directly involved in host-pathogen interactions.

Application

• Determination of N-glycans binding partners in various biological samples
• Rapid profiling of N-glycan binding properties of proteins
• Providing important information for host immunity and infectious disease
• Understanding GBP glycan interactions involved in immunity

Advantages of Us

• High sensitivity
• High reproducibility
• Multiple sample types can be detected
• Low sample consumption

CD BioGlyco offers a range of glycan microarray analysis services. These help our customers speed up their scientific research and obtain reliable data by using our microarray platform to perform highly parallel glycan expression level analysis. If you need our services, please contact us for further consultation.

Reference:

1. Arthur, C.M.; et al. Using glycan microarrays to understand immunity. Current Opinion in Chemical Biology. 2014, 18:55-61.