3-Fucosylactose (3-FL) has rich physiological activities and plays an important role in the growth and development of infants. With a strong continuous and stable supply capacity, CD BioGlyco provides you with a sufficient inventory of 3-FL and strong after-sales support.

Rich Physiological Functions of 3-FL

3-FL is an oligosaccharide found in human milk, composed of a lactose core of D-glucose and D-galactose units linked to L-fucose by α1-3 linkages. 3-FL was first separated from human breast milk in the mid-1950s and is an abundant complex carbohydrate in human breast milk, which has the function of prebiotics, immunomodulatory, and anti-pathogen adhesion therapy. At the same time, infant formula containing 3-FL may reduce diarrhea in infants.

Fig.1 The de novo pathway for 3-FL biosynthesis in metabolically engineered E. coli. (Jung, et al., 2019)

The Existence of 3-FL in Nature

It is reported that women with the Lewis blood group who lacked the gene to secrete 2'-fucosyllactose (2'FL) had significantly higher mean concentrations of 3-FL in their breast milk. The biological reasons for this variability in HMO concentrations are currently unknown, although variability is associated with multiple factors such as genetics (secretors vs non-secretors), geography, ethnicity, parity, maternal age, etc.

A recent study characterizing soluble glycans in camel milk found particularly high concentrations of oligosaccharides in colostrum and detected the presence of 3-FL. And fucosylated soluble glycans such as 3-FL and Lacto-N-fucopentaose I (LNFPI) were also found in elephant milk, but 2'FL was absent.

3-FL Production Service at CD BioGlyco

In addition to the chemical synthesis for 3-FL, CD BioGlyco mainly provides biological production methods enzymatic and microbial methods. Our Technologies include but are not limited to the following:

• Chemical synthesis
• Use a Pd/C chemical catalyst to combine fucosyl bromide with hydrogen to synthesize 3-FL.
• Enzymatic synthesis
• Selection and identification of suitable enzymes.
• High-efficiency synthesis using fucosyltransferase and cheap substrate in a one-pot method.
• Microbial methods
• Fermentation by engineered strains.
• The fed-batch cultivation of the strains.
• The metabolic pathway is optimized through genome combination, fine-tuning, overexpression, and other strategies.

Advantages of Us

• Applying various metabolic engineering strategies.
• High-yield production.
• Response specificity.
• Optimal enzyme expression levels.
• Rich resources of enzyme and bacterial resources.

CD BioGlyco accumulated a variety of 3-FL production routes to suit your needs and welcome clients from all over the world to cooperate with us. If you have any questions, please contact us directly for more details.

References:

1. Pitt, J.; et al. Safety assessment of the biotechnologically produced human-identical milk oligosaccharide 3-Fucosyllactose (3-FL). Food and Chemical Toxicology. 2019, 134:110818.
2. Jung, S.M.; et al. Production of 3-fucosyllactose in engineered Escherichia coli with α-1, 3-fucosyltransferase from Helicobacter pylori. Biotechnology Journal: Healthcare, Nutrition, Technology. 2019, 14(6): 1800498.