The high-affinity receptor 6'SLN is shared by all human influenza A and B viruses, so polymeric inhibitors that contain this protein may offer protection against all human pandemic types.

Introduction of 6'SLN

The influenza virus hemagglutinin glycoprotein binds to Neu5Ac-2-6-Gal-1-4-GlcNAc (6'-sialyl-LacNAc; 6'SLN) on the surface of host cells, starting viral infections. All human influenza A and B viruses share the high-affinity receptor 6'SLN, therefore polymeric inhibitors that include this molecule may provide defense against all human pandemic strains. Furthermore, it is presently thought that for the virus to propagate effectively from person to person, 6'SLN-terminated receptors must be recognized by the virus. If this theory is correct, 6'SLN-based medications may offer excellent defense against newly developing pandemic influenza virus strains. High molecular weight conjugates of Neu5Ac2-3Gal1-4Glc (3'SL), Neu5Ac2-6Gal1-4Glc (6'SL), and 6'SLN were produced by connecting spaced oligosaccharides to polyacrylamide (N-oxi-succinimidyl acrylate).

Fig.1 The structure of 6'SLN. (Gambaryan, et al., 2005)

Applications of 6'SLN

The interaction of the viral haemagglutinin (HA) with cell surface glycans mediates the earliest step of host cell infection by influenza A viruses (IAV). It is widely known that IAVs must bind to Gal(1,4)Glc/GlcNAc (lactose/lactosamine) glycans with a terminal (human receptors) or (avian receptors) N-acetylneuraminic residue that is often present on N-glycans. Unfortunately, little is known about the function and importance of sialylated Gal(1,3)GalNAc epitopes, which are typical O-glycoforms, in the pathogenesis of influenza viruses. Whereas 3'SLN is chosen by avian influenza strains as a key attachment site, 6'SLN is known to be exclusive to human influenza strains. Specifically binding to the hemagglutinin (HA) trimers of influenza strains are 6'SLN and 3'SLN.

6'SLN Production Services at CD BioGlyco

CD BioGlyco has a professional synthesis platform including Biocatalytic Methods, Chemoenzymatic Methods, and Chemical Methods for meeting clients' needs regarding HMO production. We have the ability to produce different scales and grades of 6'SLN. As 6'SLN has so many potential applications for human influenza strains, there are serval research hits about it. To help our clients to study the application and biological activity of 6'SLN, we built a powerful and advanced analytics platform for HMO Profiling Services.

Advantages of Us

• We have an advanced synthetic platform to produce high-quality 6'SLN.
• We have a professional service team for helping customers with questions we have the ability to solve.
• According to the client's needs, we provide different grades and scales of 6'SLN.
• Our outstanding scientists could provide professional and suitable solutions for customers' inquiries.

CD BioGlyco establishes a powerful production platform, in the field of HMO study and manufacturing, we have significant expertise and achievement. To offer our clients high-quality goods and services, we have developed a comprehensive analytic and production platform. If you are interested in our 6'SLN production service, please feel free to contact us for more detailed information.

Reference

1. Gambaryan, A.S.; et al. Polymer-bound 6'sialyl-N-acetyllactosamine protects mice infected by influenza virus. Antiviral Research. 2005, 68(3): 116–123.