CD BioGlyco provides comprehensive solutions that are tailor-made for clients. We specialize in various servicing aspects including the use of enzymatic and microbial production technologies, with the most advanced and effective production plan for 6'-Sialyllactose (6'SL).

Physiological Function of 6'SL

Human milk oligosaccharides (HMOs), the third major solid component in breast milk, are considered the first prebiotics to benefit infant health. Sialylated HMOs are an important class of HMOs, accounting for about 13% of the total HMOs. 6'SL is one of the simplest sialylated HMOs. The structures of different sialylated HMOs can be identified by nuclear magnetic resonance spectroscopy (NMRS), which is based on the specific nucleus that can form a nuclear magnetic resonance signal when absorbing the energy provided by a specific frequency radio frequency field. 6'SL is an oligosaccharide, which is essentially NeuAc bounded to a lactose molecule through α-(2, 3)- and α-(2, 6)-linkages. 6'SL showed promising prebiotic effects, especially in shaping the gut microbiota and promoting the proliferation of bifidobacteria, it also exhibits multiple health effects, including anti-adhesive antimicrobial ability, antiviral activity, regulation of intestinal epithelial cell responses, immunomodulatory activity, regulation of inflammatory response and endothelial cell permeability, promotion of brain development, and cognitive improvement.

Fig.1 Chemical structure and product ion scan spectra of 6'-SL. (Eom, et al., 2021)

6'SL Production Service at CD BioGlyco

In addition to chemical synthesis methods, CD BioGlyco is also good at using enzymatic or microbial methods to biosynthesize 6'SL. In addition, our researchers provide chemoenzymatic methods to realize the efficient synthesis of 6'SL to meet the needs of more suitable technical means for clients.

The Technical Strategies we provide include but are not limited to:

• Using sialidase and sialyltransferase with trans-sialidase activity, etc.
• Respectively or in combination expresses enzymes involved in the 6'SL synthesis pathway.
• Achieved during fermentation by metabolically engineered strains.
• One-pot reaction of multiple recombinant bacteria.

Advantages of Us

• Maintain dynamic balance in many aspects
  Multifaceted considerations balance metabolic pathways that regulate cell growth and production.
• Comprehensive elimination of bottlenecks
  Synergistically eliminating bottlenecks at thermodynamic and kinetic limits in synthetic pathways.
• Development of multiple efficient biosynthetic methods using low-cost substrates.
• Abundant biological tools
  Such as metabolite-responsive biosensors and a range of naturally available transcription factors.
• Combination of multi-disciplinary technologies
  Including protein engineering, metabolic engineering, and synthetic biology strategies and tools.

CD BioGlyco provides clients with high-quality, one-stop, time-saving 6'SL production service. If you are interested in our services, please contact us feel free.

References

1. Eom, H.Y.; et al. Development and validation of a bioanalytical method of analyzing 3'-and 6'-sialyllactose using liquid chromatography-tandem mass spectrometry in minipig plasma and its application in a pharmacokinetic study. Journal of Pharmaceutical and Biomedical Analysis. 2021, 195, 113827.
2. Zhu, Y.Y.; et al. Recent progress on health effects and biosynthesis of two key sialylated human milk oligosaccharides, 3'-sialyllactose and 6'-sialyllactose. Biotechnology Advances. 2022, 108058.