What Is A. fumigatus α-(1,3) Glucan?

The cell wall of A. fumigatus, the most prevalent respiratory human fungal pathogen, is a complex and dynamic structure that is a major source of pathogen-associated molecular patterns (PAMP). α-(1,3)-Glucan is the key amorphous polysaccharide in the cell wall of A. fumigatus, accounting for 35% to 40% of the mycelial cell wall. In A. fumigatus, α-1,3 glucan is synthesized by three α-1,3 glucan synthases (Ags1p, Ags2p, and Ags3p), which are extremely high molecular weight (>200 kDa) transmembrane enzyme. It has been reported that α-1,3-glucan is a virulence factor of A. fumigatus and that strains lacking Ags3 exhibit faster disease progression in a mouse model of aspergillosis, Ags3 affects virulence indirectly by affecting the response to environmental changes.

Fig.1 α-1,3-Glucan is a virulence factor in A. fumigatus. (Yoshimi, et al., 2017)

A. fumigatus α-(1,3) Glucan Production Services at CD BioGlyco

CD BioGlyco provides mature A. fumigatus α-(1,3) glucan production services, which mainly involve the following steps.

• Bacterial culture and fermentation

CD BioGlyco provides culture and maintenance for A. fumigatus strains, ensuring a reliable supply of starting strains required for glucan production by optimizing fermentation conditions, media formulations, and process parameters.

• Extraction and refining of glucan

CD BioGlyco carries out glucan synthesis and cell wall extraction processes to efficiently obtain A. fumigatus α-(1,3) glucan, which includes steps such as culturing cells, harvesting cells, cell wall disruption, and dextran extraction. A. fumigatus α-(1,3) glucan is then purified and refined from the extract to remove impurities and other cell wall components to obtain a high-purity glucan product.

• Quality analysis

CD BioGlyco performs precise quality control and analysis of the A. fumigatus α-(1,3) glucan products produced to ensure that meet quality standards, which includes testing for physical properties, chemical characterization, purity assessment, and microbiological testing.

Vaccine Development Services at CD BioGlyco

A. fumigatus α-(1,3) glucan is combined with appropriate carriers to improve the stability, immunogenicity, and immune response of the antigen. CD BioGlyco has established the leading Glyco™ Vaccine Development Platform, we provide design and development of antigen vectors based on A. fumigatus α-(1,3) glucan to achieve better immune effects.

Customized Services at CD BioGlyco

CD BioGlyco provides customized A. fumigatus α-(1,3) glucan products based on the needs of vaccine development, such as specific molecular weight ranges, special structural requirements, or other customized product specifications, which helps meet the specific requirements of different vaccine research projects.

Fig.2 A. fumigatusα-(1,3) glucan production and vaccine development services. (CD BioGlyco)

Applications

• Immunological: A. fumigatus α-(1,3) glucan may be applied in immunological research as an immune modulator, such as activating immune cells, and stimulating immune responses, which plays a vital role in studying immune system function and disease mechanisms.
• Anti-infective drug development: A. fumigatus α-(1,3) glucan has potential in the field of antifungal treatment. Its antigenic and immunomodulatory properties make it a candidate target for the development of novel antifungal drugs.
• Vaccine adjuvant: A. fumigatus α-(1,3) glucan is widely used as an adjuvant in vaccines to enhance the immunogenicity and immune response of vaccines, which promotes antigen presentation, stimulates immune cells and enhances immune memory.

Advantages

• CD BioGlyco is familiar with the synthetic pathways, purification methods, quality control requirements, and the latest developments in related application fields of A. fumigatus α-(1,3) glucan, which provides clients with high-quality products and professional technical services.
• CD BioGlyco has established a quality management system that complies with GMP standards to ensure that products meet consistent quality standards and regulatory requirements, which helps ensure the reliability and reproducibility of experimental results.

CD BioGlyco is equipped with advanced equipment and laboratories and has sufficient production capacity for A. fumigatus α-(1,3) glucan. We understand client needs and provide custom product specifications, technical support, and consulting services. Please feel free to contact us if you would like to obtain production service-related details.

References

1. Karnam, A.; et al. Aspergillus fumigatus cell wall α-(1,3)-glucan stimulates regulatory T-cell polarization by inducing PD-L1 expression on human dendritic cells. The Journal of Infectious Diseases. 2017, 216(10): 1281-1294.
2. Henry, C.; et al. α-1, 3 glucans are dispensable in Aspergillus fumigatus. Eukaryotic Cell. 2012, 11(1): 26-29.
3. Yoshimi, A.; et al. Function and biosynthesis of cell wall α-1,3-glucan in fungi. Journal of Fungi. 2017, 3(4).