It has been discovered that 3'SLNB, a major disaccharide, plays a substantial role in the carbohydrate structure of a number of glycoconjugates, including tumor-associated antigens.

Introduction of 3'SLNB Production Methods

The unique human milk oligosaccharide (HMO) blueprint pattern is made up of (up to) five distinct monosaccharides: D-galactose (Gal), D-glucose (Glc), D-N-acetylglucosamine (GlcNAc), D-L-fucose (Fuc), and the sialic acid D-N-acetylneuraminic acid (Sia). The overall diversity of HMO structures consists of five distinct monosaccharides and ten different kinds of connections. With the help of precise blueprint patterns, it is now feasible to synthesize a variety of HMO structures in vitro or in vivo by concentrating on a small number of distinct enzyme activities.

A significant disaccharide known as lacto-N-biose I has been found to have a significant role in the carbohydrate structure of several glycoconjugates, including tumor-associated antigens. Potential tumor markers have been detected for antibodies to lacto-N-biose I. This moiety is the main part of the Lewisa antigenic determinant. Lacto-N-biose I is 2-3-Sialylated to produce the trisaccharide 3′-sialyl-lacto-N-biose I, also known as 2,3-Sialyllacto-N-biose (3'SLNB), which has been demonstrated to be the fundamental building block of the sialyl-Lewisa, which has been discovered as a functional ligand for E-selectin. During both healthy and pathological inflammatory reactions to injury and infection, it facilitates the trafficking and recruitment of blood-borne leukocytes to endothelial cells.

Fig.1 The structure of 3'SLNB. (CD BioGlyco)

3'SLNB Production Services at CD BioGlyco

CD BioGlyco has serval production methods for 3'SLNB including Biocatalytic Methods, Chemoenzymatic Methods, and Chemical Methods, according to clients' requirements we would like to offer customized services for our clients. Due to 3'SLNB belonging to HMOs, which plays an important role in the biology process, CD BioGlyco also provides HMO Analysis Services for clients to help with their research of HMO. We have the ability to analyze HMOs with different instruments and target kinds of substances.

Advantages of Us

• We have a great service team for helping clients with any questions we could answer.
• We have an advanced synthetic platform to produce high-quality 3'SLNB production service.
• According to the client's needs, we provide different grades and scales of 3'SLNB products.
• Our outstanding scientists could provide professional and suitable solutions for customers' inquiries about HMO production and analysis.

CD BioGlyco will meet different grades of 3'SLNB production based on the advanced production department. If you are interested in our 3'SLNB production service, please feel free to contact us for more detailed information. We look forward to your inquiry and our scientists will try their best to help you solve your problems in the field of 3'SLNB research.

Reference

1. Vetere, A.; et al. Regiospecific glycosidase-assisted synthesis of lacto-N-biose I (GALΒ1-3GLCNAC) and 3′-sialyl-lacto-N-biose I (NeuAcα2–3Galβ1–3GlcNAc). European Journal of Biochemistry. 2000, 267(4): 942–949.