Analysis of TCA Cycle Metabolic Flux in Cancer Cells
Metabolic fluxomics, located at the most downstream of systems biology, is a collection of cellular metabolic reaction rates, reflecting the comprehensive effects of gene-protein-metabolite interactions on metabolic networks . The tricarboxylic acid cycle occurs in the mitochondria, and its main function is to generate reducing equivalents. At the same time, the TCA cycle is also the hub of the metabolic link between glucose, fat, and amino acids. Therefore, the metabolic flux analysis of the TCA cycle can comprehensively understand the carbon source of the TCA cycle in cancer cells and the state of the TCA cycle in cancer cells in different states. CD BioSciences uses isotope tracing combined with nuclear magnetic resonance or mass spectrometry to perform metabolic flux analysis of the TCA cycle based on the MOPCGM platform.
What We Do
We are able to systematically quantify the flux distribution of metabolic networks within cells or tissues and the relative contribution of each metabolite to the TCA cycle pathway by isotope labeling combined with nuclear magnetic resonance or mass spectrometry. We provide steady-state TCA cycle metabolic flux analysis and non-steady-state TCA cycle flux analysis. Among them, steady-state TCA cycle flux analysis has an independent marker collection mode, which takes a long time. Non-steady-state TCA cycle flux analysis takes less time but requires more time points to be analyzed. You can choose according to your needs.
For TCA cycle flux analysis, the labeling methods we can provide are as follows:
| [6-14C]-Glucose | [1-14C]-Glucose | [U-13C]-Glutamine |
| [3-13C]-Lactate | [U-13C]-Glucose | [2-13C]-Lactate |
| [U-13C]-Pyruvate | Parallel labeling |
The TCA cycle metabolites we are able to use [U-13C]-Glucose labeled are as follows, including but not limited to:
| Pyruvate | Ac-CoA | Citrate |
| Oxaloacetic Acid | Malate | Succinate |
| α-ketoglutarate |
The TCA cycle metabolites we are able to use [U-13C]-Glutamine labeled are as follows, including but not limited to:
| Oxaloacetic Acid | Malate | Succinate |
| α-ketoglutarate | Citrate |
Our Workflow
Deliverables
- Glycolytic metabolic flux analysis report
- Glycolytic metabolic flux differential analysis report
- Glycolytic metabolic flux hierarchical cluster analysis report
- Glycolysis Bi-plot analysis report
Our Advantages
- We can propose isotope tracer solutions according to customer requirements.
- We are able to analyze the metabolic fluxes of the tricarboxylic acid cycle pathway alone.
- We are able to deliver results within one month of your sample arrival.
Applications
- Uncovering tumor metabolic heterogeneity and flexibility by comparing changes in TCA cycle flux across different tumor cell subsets.
- Key metabolites can be identified by tracking the changes of metabolites in the TCA cycle pathway by TCA cycle metabolic flux technology.
CD BioGlyco can provide TCA cycle metabolic flux analysis of cancer cells from various sample sources, helping customers understand the metabolic status of the TCA cycle in real-time. Our experienced teams of scientists, researchers, and technicians provide fast turnaround, high-quality services at competitive prices for worldwide customers. Our customers have direct access to our staff and prompt feedback on their inquiries. If you are interested in our services, please contact us for more details.
