Aristichthys Nobilis Lectin Production Service

A. nobilis Lectin Extraction Service at CD BioGlyco

A. nobilis free from external damage and disease are selected, cleaned, and executed. Obtain gill tissues, wash with distilled water, and set aside at -80°C. A safe and reliable source of raw materials. Our company pays great attention to every detail of the service, all the animals are collected back, first live water feeding for some time, to confirm that the indicators are normal, and then proceed to the next step of the operation.

A. nobilis Lectin Purification Service at CD BioGlyco

The tissues are homogenized by immersion in Tris-HCl buffer and left overnight. Filtering and homogenizing, centrifugation, and collection of supernatant. We offer multiple passes through the column to collect the target lectin.

The supernatant is injected into a DEAE-agarose column for elution. Using different concentrations of Tris-HCl to elute unbound and adsorbed proteins. The ultraviolet (UV) absorbance is measured in real-time. Collection and lyophilization of target fractions.
Further purified the fraction using gel filtration chromatography, and eluted it with ultrapure water. Collect the active fraction and lyophilize it.
Furthermore, we use high-performance liquid chromatography (HPLC) to purify the collected fraction again.

A. nobilis Lectin Analysis Service at CD BioGlyco

Equipped with a professional scientific team, CD BioGlyco provides comprehensive lectin analysis services. The services we provide include but are not limited to:

Protein concentration assay service	Carbohydrate content analysis service
Effect of lectin hemagglutination activity	Homogeneity and molecular mass determination
Amino acid composition	N-terminal determination
Microbial growth inhibition assay	Microbial agglutination assay
Carbohydrate-binding specificity analysis service	Glycosidic bond analysis service

Fig.1 Schematic diagram of A. nobilis lectin production services. (CD BioGlyco)

Publication

Paper Title: Structural characterization and antitumor and mitogenic activity of a lectin from the gill of bighead carp (Aristichthys nobilis)

Journal: Fish Physiology and Biochemistry

IF: 2.9

Published: 2012

Results: The characteristic vibration peak according to the spectrum further confirmed that A. nobilis lectin (GANL) is a glycoprotein. In contrast, the alkali-treated samples show a prominent absorbance peak at 240 nm, indicating the presence of β -β-elimination, indicating that the protein and carbohydrate parts in GANL are linked by O-linkage. The inhibition of GANL varied significantly among different tumor cell lines. HeLa, SKOV 3, and HepG 2 are inhibited by GANL. In contrast, GANL stimulated proliferation in the LoVo cell line.

Fig.2 The mapping of FT-IR, UV, and cellular antiproliferative analysis. (Yao, et al., 2012)

Applications of A. nobilis Lectin

A. nobilis lectin has cytotoxic activity against human cancer cells and mitotic activity against mouse splenocytes.
A. nobilis lectin plays an important role in the innate immune response of animals and is a potential cancer therapeutic agent.
A. nobilis lectin plays key physiological roles as recognition molecules within cells, between cells, or between organisms.

Advantages of Us

The purification service, from filler to elution gradient, has been optimized countless times, only to give the clients the best service.
Our company has successfully provided Lectin Production Services to clients in many countries.

CD BioGlyco is a professional company specializing in the field of glycobiology. We offer different kinds of lectin production services, including but not limited to Plant Lectins, Animal Lectins, and Fungal Lectins. We provide professional lectin production services to clients all over the world. Please feel free to contact us for further information.

References

Yao, D.; et al. Structural characterization and antitumor and mitogenic activity of a lectin from the gill of bighead carp (Aristichthys nobilis). Fish Physiology and Biochemistry. 2012, 38(6): 1815-1824.
Pan, S.; et al. Isolation and characterization of a novel fucose-binding lectin from the gill of bighead carp (Aristichthys nobilis). Vet Immunol Immunopathol. 2010, 133(2-4): 154-64.

This service is for Research Use Only, not intended for any clinical use.