Bauhinia purpurea Lectin (BPL) Production Services at CD BioGlyco

Lectins are a class of non-enzymatic, non-antibody glycan-binding proteins that are widely distributed in animals, plants, and microorganisms. CD BioGlyco is dedicated to Lectin Production, which contains Plant Lectins, Animal Lectins, and Fungal Lectin. We have professional BPL production service in plant lectin production.

• Extraction and purification
  • First, we grind B. purpurea seeds and use saline extraction. Then, the supernatant is concentrated and then subjected to salting out. Next, the precipitate is taken and desalted using a dialysis operation. Finally, lyophilization is performed to obtain the crude lectin.
  • We acidify the crude lectin product. The pH is adjusted by adding a buffer to it, followed by column chromatography to wash out the heterogeneous proteins and collect the target product. Finally, pure BPL is obtained by dialysis to remove glycans and lyophilization.
• Performance characterization
  • Molecular weight and subunit molecular weight
    The molecular weight of lectin is determined by gel permeation chromatography, and the molecular weight of the subunit is determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
  • Structure
    The primary, secondary, and tertiary structures of BPL are determined using circular dichroism.
  • Isoelectric point
    The isoelectric point of BPL is obtained by measuring the pH of each part in an amphoteric electrolyte carrier with an acidimeter and comparing it with the stained protein bands.
  • Sugar content
    Glucose is used as the standard sugar and measured by anthrone colorimetry.
  • Agglutination activity
    After doubling dilution on the coagulation plate, add a small amount of glycan solution to each hole, then add erythrocyte suspension, mix and let it stand, and observe the agglutination activity using a microscope.
  • Stability
    Thermal stability: Sampling at different temperatures to check the agglutination activity.
    Acid-base stability: The samples are kept warm in different buffers and then the agglutination activity is determined.

In addition, we will determine the sugar-binding specificity of BPL. It is found to have a binding affinity for d-galactose and d-glucose patterns.

Fig.1 Specifics of the production of BPL. (CD BioGlyco)

Moreover, we offer the lectin products as shown in the table below.

Table 1 BPL product list.

Publication

Applications

• B. purpurea lectin production is used in the development of anticancer agents.
• B. purpurea lectin production is used as a way to better understand inflammation.
• The uptake of lectins by different insects interferes with some biological processes, so B. purpurea lectin production is used in the development of insecticides.

Advantages

• Our lectins are highly selective and stable.
• We have a strong resource platform to provide a one-stop service from extraction to analysis.
• We have world-leading lectin production technology to ensure client satisfaction.

CD BioGlyco is constantly updating technology in the field of lectin research to provide systematic lectin production services to our clients, and we offer custom research protocols according to our client's research needs. All our programs are quality-tested. We also hope to be our clients' right-hand man in the field of lectin research. Please feel free to contact us if you are interested in our services and have any questions.