Bioorthogonal Aldehyde-amine Reaction-based Cell Surface Chemical Conjugation Glycoengineering Service

Bioorthogonal Aldehyde-amine Reaction

The bioorthogonal aldehyde-amine reaction consists of sugar-protein conjugation through Schiff base formation between sugar-derived aldehydes and amines in proteins followed by a reduction. This reaction includes the initial formation of bonds between carbonyl groups in carbohydrates and amino groups in proteins via Schiff bases. The Schiff base was then specifically reduced to the more stable amine with the weak reducing agent sodium cyanoborohydride.

Fig.1 Conjugation by reductive amination. (Morais & Suarez, 2022)

Bioorthogonal Aldehyde-amine Reaction-based Cell Surface Chemical Conjugation Glycoengineering Service at CD BioGlyco

CD BioGlyco offers bioorthogonal aldehyde-amine reaction-based cell surface chemical conjugation glycoengineering service to help researchers to decipher cell surface glycobiont in detail. From service selection to report and biological interpretation, our experts help you in every step.

• Conjugation of natural polysaccharides and synthetic carbohydrates.
• Solid-phase automated oligosaccharides synthesis method, chemoenzymatic approaches, and one-pot protocols are used to conjugate natural polysaccharides and synthetic carbohydrates.
• Conjugation of glycan protein.
• Conjugation to nanoparticle carriers.
• Including outer membrane vesicles (OMVs), virus-like particles (VLPs), ovalbumin peptides, d-glucose, and liposomes.
• Glycans imaging
• Imaging glycans helps to understand the structure, localization, and function of glycans in cells through bioorthogonal chemistry.
• Metabolic labeling of cell-surface glycans
• Introduce artificial recognition markers to enable the visualization and tracking of glycans in cells, tissues, and living organisms.
• The visualization in living systems is achieved by metabolic labeling of cell surface glycans followed by bioorthogonal reaction with imaging probes.
• The advent of metabolic labeling using bioorthogonal chemical reporters and small molecule fluorescent reporters has enabled the study and targeting of specific bacterial glycans in their native environment.

Fig.2 Bioorthogonal aldehyde-amine reaction-based cell surface chemical conjugation glycoengineering. (CD BioGlyco)

Applications

• Based on the glycan labeling methods, strategies enabling cell-selective and tissue-specific glycan labeling and protein-specific glycan imaging have been developed.
• Reductive amination has been widely used in glycoconjugate synthesis due to its simplicity.
• Chemical conjugation could combine biomacromolecules including proteins (antibodies, antigens) and nucleic acids, as well as small molecule probes such as fluorescent and biophysical probes.

Advantages

• The major features of the bioorthogonal aldehyde-amine reaction include outstanding reliability, specific selectivity, and good compatibility with naturally occurring functional groups, making them a powerful tool for studying the structure and function of viruses.
• Bioorthogonal chemistry promotes the understanding of the structures and biological functions of glycans.
• Our services help to image glycan dynamics, assay and inhibit glycan biosynthesis, and profile glycoproteins and glycan-binding proteins.
• With this method, researchers are now better poised than ever to dissect the biological function of glycans in physiological or pathological contexts.

CD BioGlyco offers high-quality Cell Surface Chemical Conjugation Glycoengineering Services for clients worldwide. Our expert team has extensive experience in the field of glycobiology research. If you are interested in our services, please feel free to contact us for more information.