Protein Derivative of Brucellin (BR-PPD) Evaluation Service

Your Path to Precision: Protein Derivative of Brucellin (BR-PPD) Evaluation Perfected

BR-PPD is a purified protein derivative made from the supernatant of Borrelia burgdorferi after culture, sterilization, and removal of the bacterium. It is mainly used for clinical diagnosis of Brucellosis and monitoring of immune response after vaccination with the Brucella vaccine. CD BioGlyco has a professional Biological Evaluation team to provide reliable BR-PPD evaluation services relying on a high-quality analytical system. Moreover, we provide high-quality Pharmaceutical Analysis and Pharmaceutical Excipient Analysis.

Our Pharmaceutical and Biological Analysis team comprehensive BR-PPD evaluation including bulk test, final bulk test, and final product test services.

Bulk test
- Purity analysis
  - Protein: Our staff selects proteins with the same or similar structure as the proteins of the variety to be measured as a control, and carries out the determination of the protein content in the samples through Kjeldahl, Lowry, biuret, bicinchoninc acid procedure (BCA), coomassie blue staining or ultraviolet-visible spectrophotometry (UV-Vis).
  - Polysaccharides: The bulk, glucose standard solution and anthrone mixture are mixed and boiled in a water bath for 20 minutes. We measure the absorbance at a wavelength of 620 nm and calculate the polysaccharide content based on the change in glucose concentration and absorbance
  - Nucleic acid: We use UV-Vis spectrophotometry to measure the absorbance at 260 nm to calculate the nucleic acid content.
- Potency assay
  - Our researchers dilute the sample and standard and inject it into guinea pigs to observe the longitudinal and transverse diameters of localized sclerosis. Notably, the choice of dilution is the key to potency calculation. Our careful calculations and many years of experience help our clients to get the best results.
- Safety analysis
  - Our team provides high-level sterility testing and abnormal toxicity test services to clients.
  - Sensitization: We set up safe and healthy experimental and control animals. The experimental group is inoculated with this sample 3 times according to the cycle and then inoculated with this product once together with the control group. Our staff observe the difference between the two groups of animals and record.
Final bulk test and final product test
- Identification
  - We determine potency by injecting the sample into guinea pigs sensitized to B. abortus and observing the longitudinal and transverse diameters of local sclerosis.
- Phenol content
  - We determine the phenol content by repeated titrations with sodium thiosulfate.
- Others
  - We provide accurate pH tests, sterility tests, and abnormal toxicity tests.

Schematic diagram of BR-PPD assessment. (CD BioGlyco)

Publication Data

Technology: Liquid chromatography-tandem mass spectrometry (LC-MS/MS), Capillary electrophoresis, Real-time PCR

Journal: Frontiers in Immunology

Published: 2022

IF: 7.3

Results: In this work, researchers used mass spectrometry-based, label-free, relative quantitative proteomics to identify differentially expressed (DE) proteins between Brucella strains Y3 and M5 and to analyze differences in virulence between the two strains in vitro and in vivo. The results showed that a total of 147 DE proteins were identified. Among them, three strains of vjbR proteins were expressed twice as much as the M5 strain. Notably, many of the specific proteins serve as a key to differentiate vaccinated animals from wild-type infected animals.

Fig.1 Biological function and subcellular localization scale analysis of differentially expressed proteins. Fig.1 Classification of gene ontology secondary annotation and subcellular location of DE proteins. (Zhang, et al., 2022)

Applications of BR-PPD Evaluation

BR-PPD strain differential proteome expression analysis leads to a better understanding of the underlying mechanisms of differences in toxicity.
BR-PPD evaluation promotes vaccine development and the discovery of new therapeutic targets.
BR-PPD evaluation provides valuable information on the biology and determinants of pathogenicity.

CD BioGlyco pays extra attention to the quality of our services. Our goal is to be your first choice in pharmaceutical and biological analysis. Don't hesitate to contact us whenever you need assistance.

Reference

Zhang, H.; et al. Using a relative quantitative proteomic method to identify differentially abundant proteins in Brucella melitensis Biovar 3 and Brucella melitensis M5-90. Frontiers in Immunology. 2022, 13: 929040.

This service is for Research Use Only, not intended for any clinical use.