Bubalus Bubalis Lectin Production Service

B. bubalis lectin (BBL) is a soluble β-galactoside binding lectin with the highest affinity for lactose and other glycol groups in the glycosidic form. With a molecular weight of 14.2 kDa, the neutral sugar content of the soluble lectin is estimated to be 3.3%. CD BioGlyco provides comprehensive and thoughtful Lectin Production Services to help advance client research into the diversity, structure, and biological role of BBL.

BBL Extraction and Purification Service at CD BioGlyco

CD BioGlyco has many years of successful extraction and separation experience. Selection of fresh nerve tissue, wash, remove meninges, and cut into small pieces. The tissues are homogenized in phosphate compound buffer and centrifuged to obtain supernatant. A mixture of ammonium sulfate precipitation is added to the supernatant, followed by gel filtration to collect target components. Remove the impurities from the secondary dialysis protein peak components.

BBL Analysis Service at CD BioGlyco

In addition, we offer a range of analytical methods for the purified lectin. Including but not limited to the following:

Determination of hemagglutination activity: We provide enzymatic animal erythrocytes BBL agglutination services. Add PBS, shake well, and leave at room temperature for some time before detection after serial dilution with the U-type microtiter plate.
Carbohydrate specificity analysis service: Different types of carbohydrates and their derivatives are serially diluted in the buffer. Observe agglutination after adding purified BBL and enzyme-enriched erythrocyte suspension.
Molecular weight determination: We use acrylamide gels for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) determination and molecular weight determination. The molecular weight of the Native Lectin is determined by size-exclusion chromatography of the gel column.
Chemical analysis: Determination of carbohydrate content of purified lectins by phenol-sulfuric acid reaction.

Fig. 1 Schematic diagram of BBL analysis service. (CD BioGlyco)

Publication

Paper Title: Physicochemical properties and oxidative inactivation of soluble lectin from water buffalo (Bubalus bubalis) brain

Journal: Neurochemical Research

IF: 4.4

Published: 2008

Results: The lectin purified from the B. bubalis gave a single polypeptide band of 14.5 kDa corresponding to it under SDS-PAGE reducing conditions. Suggesting that it was composed of two identical noncovalent-linked 14.5 kDa subunits. The structure of the dimer was further confirmed by the Scatchard analysis, indicating the presence of two carbohydrate-binding sited for each native lectin molecule. It was also shown that the oxidized form was completely inactive, thus demonstrating that oxidative functional lectins in neural tissue may simply act as an autocrine or paracrine factor, and function more like a cytokine or chemokine.

Fig.2 Analytical mapping of BBL. (Rizvi&Banu, 2008) Fig.2 Analytical mapping of BBL. (Rizvi & Banu, 2008)

Applications of BBL

BBL plays an important role in the development of brain tumors and potential treatment.
BBL is a potent inducer of cell aggregation in mammalian brain cells.
BBL is involved in neurotransmission processes and neuronal cell differentiation.

Advantages of Us

Our company pays extra attention to the purity of BBL, and in the extraction and purification, we remove impurities many times to ensure high-quality production services.
Our talented team of professionals has sufficient background knowledge and years of service experience to meet a wide range of clients' needs.

CD BioGlyco provides high-quality Animal Lectin production services. We are recognized by our clients around the world for our comprehensive and attentive services. Please feel free to contact us if you would like to learn more about the production of BBL.

References

Capparelli, R.; et al. Mannose-binding lectin haplotypes influence Brucella abortus infection in the water buffalo (Bubalus bubalis). Immunogenetics. 2008, 60(3-4): 157-65.
Rizvi, S.; Banu, N. Physicochemical properties and oxidative inactivation of soluble lectin from water buffalo (Bubalus bubalis) brain. Neurochem Res. 2008, 33(3): 468-76.

This service is for Research Use Only, not intended for any clinical use.