What Is C. albicans Mannan?

C. albicans mannan is a polysaccharide molecule present in the cell wall of C. albicans. It is a polysaccharide structure formed by multiple mannose units through a specific connection method and has a complex chemical structure and variability, which makes it in the host play important biological functions in pathogen interactions. Studies have shown that C. albicans mannan may serve as an immunogen for immune effector organs in inducing antibody production, activating immune cells, and regulating immune inflammatory responses. Therefore, it is of great significance in the fields of immunological research, vaccine development, and antifungal treatment.

C. albicans Mannan Production Service at CD BioGlyco

• C. albicans mannan extraction and purification services
  CD BioGlyco provides extraction and purification of mannan from C. albicans.
  • Cultivation and cell harvesting of C. albicans
    CD BioGlyco carries out large-scale cultivation of C. albicans under appropriate culture conditions to obtain a sufficient number of cells for the extraction of mannan.
  • Extraction of mannan
    C. albicans cultures undergo a series of processing steps such as cell disruption, washing, and purification to extract mannans. This process may use physical methods (such as ultrasonic waves or high-pressure homogenization) or chemical methods (such as enzymatic hydrolysis or solvent extraction) to break up the cells and release mannan.
  • Purification
    The extracted mannan requires further purification to remove impurities and other molecules, such as filtration, centrifugation, gel filtration chromatography, or solvent precipitation.
• Mannan quantitative analysis services
  CD BioGlyco analyzes provided C. albicans mannan samples using appropriate analytical techniques to evaluate mannan content, purity, and structure.
  • Spectrometry
  • High-performance liquid chromatography (HPLC)
  • Mass spectrometry (MS)
  • Enzyme-linked immunosorbent assay (ELISA)
• Vaccine adjuvant optimization services
  CD BioGlyco has established a variety of specialty platforms for Carbohydrate-based Adjuvant Development. We provide optimization for integrating C. albicans mannan with an immune adjuvant into vaccine formulations. By optimizing factors such as mannan content, form, and delivery method in the vaccine formulation, the immunogenicity and protective efficacy of the vaccine are improved.
• Antibody preparation services
  Mannan is applied as an antigen to induce the production of antibodies specific against mannan. These antibodies are used to detect, quantify, and characterize mannans in research and as evaluation tools during vaccine development.

Fig.1 C. albicans mannan production services. (CD BioGlyco)

Applications

• Immunological research: C. albicans mannan, as one of the main antigens of C. albicans, is used for in-depth immunological research on the interaction between mannan and the immune system, immune response mechanism, and other aspects.
• Vaccine development: C. albicans mannan may be used as a vaccine candidate for the prevention of C. albicans infectious diseases. Mannans may be used as antigenic components in vaccine development to induce immune responses and provide protection.
• Diagnostic reagent development: Mannan is applied to detect and identify markers of C. albicans infection, such as antibody determination, immunochromatography test strips, etc.
• Drug research and development: C. albicans mannan’s manufacturing services are available in the field of drug research and development, which will help to understand the biological characteristics of C. albicans to develop new drugs or research for C. albicans infection.

Advantages

• CD BioGlyco has technical experts and teams who are proficient in the production methods of C. Albicans mannan and have rich professional knowledge and experience.
• CD BioGlyco provides high-quality C. albicans mannan products through strict quality control processes and analytical techniques to ensure the consistency of its purity, structure, and functionality.

CD BioGlyco has established a powerful Glyco™ Vaccine Development Platform, and we provide efficient and professional C. albicans mannan production and vaccine development services. Please feel free to contact us if you need to inquire about specific details.

References

1. Bystrický, S.; et al. Candida albicans mannan–protein conjugate as a vaccine candidate. Immunology Letters. 2003, 85(3): 251-255.
2. Hall, R.A.; Gow, N.A. Mannosylation in Candida albicans: role in cell wall function and immune recognition. Molecular Microbiology. 2013, 90(6): 1147-1161.