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C. neoformans α-(1,3) Glucan Production Service

C. neoformans α-(1,3) Glucan Production Service at CD BioGlyco

C. neoformans is an opportunistic fungal pathogen of humans that can be obtained from the environment. Its most important virulence factor is its polysaccharide capsule, which can be used in diagnostic tests to identify cryptococcus antigens (CrAg). CD BioGlyco has long specialized in C. neoformans α-(1,3) glucan production.

Synthesis method
For the synthesis of C. neoformans α-(1,3) glucan antigens, we synthesize specific glucans using organic enolic acids and anhydrides as reaction substrates.
Immunological test
- The biological properties as well as the serotype specificity of C. neoformans α-(1,3) glucan antigen are determined by the physical properties and chemical structure of the polysaccharide antigens that make up the capsule. Therefore, we provide structural analysis as well as performance characterization of polysaccharide antigens. Specific techniques include but are not limited to, nuclear magnetic resonance (NMR), infrared spectrum (IR), mass spectrometry, and others.
- Secondly, we test the immunizing effect of the antigen on host cells. Among the testing methods, the CrAg lateral flow assay (LFA) is a dipstick immunochromatographic assay with high sensitivity and specificity.
Vaccine development
Cryptococcosis is a systemic fungal infection caused by C. neoformans, and cryptococcal infections are usually confined to the lungs. And C. neoformans α-(1,3) glucan antigen produces specific expression when infection occurs in humans. Therefore, we use C. neoformans α-(1,3) glucan as an antigen in combination with adjuvants, stabilizers, and surfactants for the development of antifungal vaccines.

Fig.1 We offer ranging from antigen production to vaccine development services. (CD BioGlyco) Fig.1 We offer ranging from antigen production to vaccine development services. (CD BioGlyco)

Publication

Technology: Chemical synthesis of α-(1,3) glucan

Journal: Polymer International

IF: 3.2

Published: 2020

Result: α-(1,3) glucan and its ester derivatives with C=C structure were synthesized using 3-butenoic acid and TFAA as reaction materials. Their chemical structures were then characterized by NMR and found to be converted to 2-butyl during esterification. Meanwhile, the surface of the cast film of the ester was modified using three different thiol compounds. The PFD surface modification improved the WCAs of the film with high water repellency, and the modification with MPD resulted in more hydrophilicity.

Applications

C. neoformans α-(1,3) glucan can be used in the development of antifungal vaccines.
C. neoformans α-(1,3) glucan can be used to characterize fungal infections in humans.
C. neoformans α-(1,3) glucan can be used in the development of polysaccharide antigens.

Advantages

Our production method is efficient and fast and can be synthesized in large quantities at the same time.
We have a professional team to provide a one-stop solution for the production of antigens and the development of vaccines.

CD BioGlyco has a specialized research team and many years of experience in glucan antigen production. If you are interested in our services, please feel free to contact us for more details without any hesitation.

Reference

Hori, Y.; et al. Synthesis of α-1,3- and β-1,3-glucan esters with carbon-carbon double bonds and their surface modification. Polymer International. 2020, 70(5): 573-581.

This service is for Research Use Only, not intended for any clinical use.

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