C. neoformans Glucuronoxylomannan (GXM) Production Service at CD BioGlyco

CD BioGlyco has constructed a Vaccine Research and Development Platform and has long been committed to the development of various vaccines and the production of related antigens. Among them, GXM has a significant recognition effect on certain monoclonal antibodies, while GXM-protein conjugated vaccines can trigger protective immune responses. Therefore, we provide not only the production of GXM but also the immunization study of GXM and the development service of related vaccines.

• Synthesis of GXM

We synthesize the target antigen by using a specific glycoside as a substrate for coupling with the pentasaccharide receptor, through a process of glycosylation, protection-deprotection, and concomitant reduction of the azide group to form the amino group required for subsequent coupling to the protein. During this process, the target antigen is isolated, purified, and structurally analyzed using nuclear magnetic resonance (NMR) and chromatographic techniques.

• Immune response testing and vaccine development

Given the importance of podocarp polysaccharides in virulence and the preference for polysaccharide-based vaccines for encapsulated bacterial pathogens, we inject the GXM antibody into a mouse infection model to probe the immune response. In this process, the coupling of C. neoformans podoplanar polysaccharides to proteins produces highly immunogenic compounds that can trigger high antibody responses.

Based on the results of the immune response tests, we will develop an antifungal vaccine consisting of a podoplanar polysaccharide-protein complex that will be highly immunogenic.

Fig.1 We offer a full portfolio from antigen production to vaccine development. (CD BioGlyco)

Applications

• GXM can be used for diagnosis, development of antifungal vaccines, and treatment of HIV.
• GXM can be used to characterize specific bacterial infection diseases.
• GXM can be used in the development of monoclonal antibodies.

Advantages

• The services we offer include full production of antigens, related immune response testing, and vaccine development, which greatly saves our clients' time.
• All of our services have undergone numerous quality tests and are skillfully mastered at every step.
• We have a professional research team to provide timely and useful assistance to our client's needs and inquiries.

CD BioGlyco has specialized knowledge and extensive experience in the field of antigen production and vaccine development. We not only provide our clients with the production of antigens but also vaccine development. Our team is knowledgeable and capable of meeting your requirements. If you are interested in our services, please feel free to contact us for more details.

Reference

1. Oscarson, S.; et al. Synthesis and immunological studies of glycoconjugates of Cryptococcus neoformans capsular glucuronoxylomannan oligosaccharide structures. Vaccine. 2005, 23: 3961-3972.