Properties
Description
Canine lipopolysaccharide-binding protein (LBP) ELISA kit, an enzyme-linked immunosorbent assay, has the ability to detect levels of canine LBP as low as 1 μmol/L and its detection range is 7.5-240 μmol/L.
Assay Principle
Canine lipopolysaccharide-binding protein (LBP) ELISA kit utilizes purified canine LBP antibody to coat microtiter plate wells, creating a solid-phase antibody. LBP samples are then added to the wells, forming an antibody-antigen-enzyme-antibody complex with HRP-labeled antibodies. After thorough washing, a TMB substrate solution is added, resulting in a blue color formation catalyzed by the HRP enzyme. The reaction is stopped by adding a sulfuric acid solution, and the color change is measured using a spectrophotometer at a wavelength of 450 nm. The concentration of LBP in the samples is determined by comparing the optical density (O.D.) of the samples to a standard curve.
ELISA Type
Sandwich ELISA kit
Assay Type
Sandwich ELISA kit
Species Reactivity
Canine
Components
Closure plate membrane; Sealed bags; Micro ELISA stripplate; Standard; HRP-Conjugate reagent; Sample diluent; Chromogen solution A; Chromogen solution B; Stop solution; Wash solution
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Method
Colorimetric, OD 450 nm
Assay Range
7.5-240 μmol/L
Precision (Intra-Assay)
CV <10%
Precision (Inter-Assay)
CV <15%
Cross-reactivity
No significant cross-reactivity or interference was observed.
Applications
Canine lipopolysaccharide-binding protein (LBP) ELISA kit can be used to measure the level of canine LBP in serum, plasma, tissue homogenate and other biological fluid samples.
