Perfecting Caramel Analysis: Opt for Dependable Outcomes

CD BioGlyco's caramel analysis service offers a comprehensive and customized solution for assessing the safety and quality of caramel samples. We ensure that our results of Pharmaceutical Excipient samples are accurate through diverse advanced analysis approaches. Caramel is primarily derived from carbohydrates such as sucrose or glucose through heating processes. It is a dark brown viscous liquid or dark brown powder with a slight peculiar odor. Caramels are soluble in water and form a homogeneous mixture. It dissolves in ethanol with a concentration of less than 55% (v/v) but is insoluble in ether, chloroform, acetone, benzene, or n-hexane.

• Purity

We will dilute the sample with water, add phosphoric acid, and ensure thorough mixing. Ideally, there should be no formation of precipitate.

• Absorbance

The sample will be dissolved in water to prepare a solution, ensuring thorough dilution. We utilize (ultraviolet) UV-visible spectrophotometry to measure the absorbance at 610 nm wavelength.

• 4-Methylimidazole

Concerns are raised about the possible formation of 4 dimethylimidazole during caramel production. Due to its carcinogenic potential, the use of caramels is being investigated. The risk of cancer could be increased by exposing consumers to the 4-methylimidazole. Therefore, we will analyze the presence of 4-methylimidazole in caramel and determine the content of 4-methylimidazole by high-performance liquid chromatography (HPLC). By this analysis, we provide accurate data for this important parameter.

• Ammonia nitrogen

Ammonia nitrogen, an important test item, will be detected by using the titration method. We will place the sample in a distillation flask containing magnesium oxide and water. Then, recover the distillates into a boric acid solution. The ammonia nitrogen content is determined by titrating the distillate with hydrochloric acid and measuring the absorbance.

• Sulfur dioxide

We will detect sulfur dioxide by quantifying its presence in a sample through a titration process using sodium hydroxide. Through the method, the concentration of sulfur dioxide is assessed in the sample accurately.

• Ash content

The ash content will be determined by grinding the sample, mixing it well, and then taking a portion. We will place it into a crucible, heat it gradually to a high temperature until it completes ashing, and calculate the ash content based on the residual weight.

• Heavy metal
  • Lead
    Atomic absorption spectrophotometry is a method based on the absorption of electromagnetic radiation by atoms at specific wavelengths. We employ this method for the quantitative analysis of metals and certain nonmetals in samples, such as lead and arsenic. The metals in the sample are first converted into a gaseous atomic state by this method. The light source of special wavelength excites the gas atoms in the sample, which causes them to reflect it at these wavelengths. The intensity of the absorbed light is measured to reflect the concentration of the metals in the sample.
    
    • Sample preparation: The sample will be converted to a gaseous atomic state, typically by the use of flame atomization or other chemical techniques.
    • Atomization: A light source of specific wavelengths excites the sample in gaseous atomic form, causing it to absorb light at these wavelengths.
    • Measurement of absorption: We determine the concentration of lead by measuring the intensity of light absorbed by the sample.
    • Comparison and calculation: The absorbance of the sample will be compared to that of standard or control samples to calculate the concentration of metals in the sample.

• Arsenic

For the detection of arsenic salts, we use the Gutzeit method and the dithizone method. We will achieve a complete detection of arsenic with the combination of these two methods. The Gutzeit method provides sensitivity for trace levels, while the dithizone method offers specificity and efficiency in screening. In this dual approach, the detection of arsenic salts in a wide range of concentrations is guaranteed to be reliable and accurate.

Advantages

• Our analysis service covers various aspects such as purity, absorbance, and detection of specific compounds like 4-methylimidazole, ammonia nitrogen, sulfur dioxide, ash content, and heavy metals including lead and arsenic salts.
• We employ diverse analytical techniques such as UV-visible spectrophotometry, HPLC, titration methods, and specific chemical assays to ensure accurate and reliable results.
• We provide detailed and clear analysis reports, presenting the various data and results in a comprehensible manner.

CD BioGlyco uses advanced techniques in analysis service that also covers Pharmaceutical and Biological samples. We enable precise identification and quantification of key components and our data facilitates clients in gaining a comprehensive understanding of their samples. Now contact us and our experienced analysis team will provide comprehensive solutions and personalized recommendations to meet your specific needs.