Nanotechnologies have contributed to the realization of glycopeptide enrichment methods, especially from complex samples in preparation for MS characterization. CD BioGlyco uses nanomaterials with various properties and morphologies such as nanoparticles, mesoporous materials and nanotubes to help customers improve the sensitivity and specificity of glycopeptide separation.

Carbon Nanotubes

Carbon nanotubes (CNTs) are allotropes of carbon and possess cylindrical nanostructures. They have excellent mechanical, thermal, electrical and optical properties, and are used individually or in combination to provide keen sensors or on the other hand multifunctional materials. In addition, they have high angle proportions that are well suited for long and durable detection. Therefore, CNTs are widely used in the electronics industry, optical industry and other fields of materials science and technology.

Functionalized CNTs are applied in biology as biosensing platforms and biocomposites, and both applications enhanced glycan signal detection. The former (electrochemical biosensor) has shown high sensitivity, selectivity and fast response in analyzing mannose in human lung cancer cells. Previous studies have developed a new lectin-based electrochemical biosensor constructed from functionalized multi-walled CNTs for competitive determination of glycan expression on living cells. With the selection of more lectins with the biosensor advancement, this strategy has promising applications in the assay of other glycans on living cancer cells.

Fig.1 A schematic representation of (A) the preparation procedure of the Con A-based biosensor and (B) the preparation of the {CNT/ thionine/Au–S–mannose} biocomposites. (Zhang, 2011)

Our Strategies

To meet the scientific requirements of our customers, CD BioGlyco has established a variety of highly specific glycopeptide enrichment methods based on CNTs materials. The strategies we offer include but are not limited to:

• We developed a strategy for the stepwise synthesis of boronic acid-functionalized magnetic carbon nanotubes (MCNTs) that allows for highly specific glycopeptide enrichment even in the presence of large amounts of interfering non-glycopeptides.
• A novel lectin-based functionalized multi-walled CNTs electrochemical biosensor was constructed to capture glycans from living cells.
• Thiomannosyl dimer was synthesized to construct CNT/thirosine/Au-S-mannose. This biocomposite utilizes CNTs as carriers to load large amounts of thionine to generate electrochemical signals and AuNPs to anchor the thiomannosyl dimer.

Applications

• Empowering traditional MS-based technologies
• Development of biosensors and biocomposite
• Early-stage disease detection
• Promote research in integrated areas of nanoengineering, mass spectrometry and glycoproteomics

Advantages of Us

• Rigorous research attitude and strong work ethic
• Expanding expertise based on the latest scientific research achievements
• Constantly updated research equipment
• Detailed after-sales service

CD BioGlyco offers a variety of prospective selective enrichment platforms for precise glycoproteomics analysis, and we will provide the most suitable strategy and service according to our client's needs and submit a detailed evaluation report. Please contact us for further consultation.

Reference

1. Zhang, X.; et al. Lectin-based electrochemical biosensor constructed by functionalized carbon nanotubes for the competitive assay of glycan expression on living cancer cells. Chemical Science. 2011, 2(12):2353.