Delivering Precision in Carboxymethylcellulose (CMC-Na) Sodium Analysis

CMC-Na is an organic substance with the chemical formula [C₆H₇O₂(OH)₂OCH₂COONa]_n. It is an anionic polymer compound produced by the interaction of cellulose with sodium monochloroacetate under alkaline conditions and is a white to slightly yellow fibrous or granular powder. With molecular weights ranging from thousands to millions, CMC-Na is easily dispersed in water to form transparent colloidal solutions and is the predominant ionic cellulose gum. In addition, CMC-Na is often used as a pharmaceutical excipient, providing different functional supports for a wide range of formulations, from oral solid dosage forms (granules or pellets) to powder and tablet coating.

Here, CD BioGlyco provides high-quality CMC-Na analysis services based on our rich experience in Pharmaceutical Excipient Analysis.

• Identification
  The colloidal solution of CMC-Na has the following special properties, we use them to identify whether the sample is CMC-Na or not.
  
  • When copper sulfate is added to a colloidal solution of CMC-Na, a blue flocculent precipitate is produced.
  • Adding an equal volume of barium chloride solution to a colloidal solution of CMC-Na produces a white precipitate.
  • A colloidal solution of CMC-Na undergoes a flame reaction of the sodium salt.
• Solubility
  CMC-Na dissolves into a gelatinous solution in water but is insoluble in ethanol, ether, or trichloromethane.
• Viscosity
  After making CMC-Na into a colloidal solution, the viscosity of CMC-Na is checked by using a rotational viscometer.
• pH
  After making a colloidal solution of CMC-Na, we use a pH meter to test its acidity and alkalinity. The pH value of CMC-Na should be 6.5-8.0.
• Degree of substitution
  After washing CMC-Na several times with ethanol, it is burnt over low heat until it chars. Next, it is dissolved with water and sulphuric acid and titrated with sodium hydroxide solution as a titrant and methyl red as an indicator solution. The titration is stopped when the red color of the whole solution disappears. Finally, the degree of substitution of CMC-Na can be calculated based on the amount of titrant used. The degree of substitution of CMC-Na should be 0.59-1.00 according to the dry product.
• Content
  The sample is dissolved by glacial acetic acid and titrated with a perchloric acid titrant according to the potentiometric titration method. The content of the sample can be calculated based on the amount of perchloric acid titrant used. For example, each 1 ml of perchloric acid titrant (0.1 mol/L) is equivalent to 2.299 mg of Na.
• Chloride
  A dilute nitric acid and silver nitrate solution are added sequentially to a diluted colloidal solution of CMC-Na and a standard sodium chloride solution. Afterwards, compare the phenomena produced by both of them. If the phenomena are the same, it means that CMC-Na contains chloride.
• Sulphate
  A barium chloride solution is added to both the CMC-Na colloidal solution and the standard potassium sulfate solution after adding the appropriate amount of dilute hydrochloric acid to each. Next, place both on the same black background for observation. If the phenomena are consistent, it means that CMC-Na contains sulfate. It is worth noting that the CMC-Na solution must not be thicker than the standard potassium sulfate solution.
• Sodium ethanoate
  Acetic acid, acetone, and sodium chloride are added to the CMC-Na solution and stirred until the CMC is completely precipitated. Then the precipitate is filtered and dissolved with acetone, and 2,7-dihydroxynaphthalene sulphuric acid solution is added to the solution. The absorbance is measured at the wavelength of 540 nm according to the ultraviolet-visible (UV) spectrophotometric method, and the content of sodium ethanoate in CMC-Na is obtained. It is worth noting that the whole operation must be carried out under the protection of light.
• Loss on drying
  CMC-Na is dried at 105°C for 6 hours and the weight loss should not exceed 10.0%.

Frequently Asked Questions

• How many types of CMC-Na can be classified?
  There are two major types of products, alkaline and neutral, depending on the application:
  
  • Alkaline CMC-Na has a viscosity value of 0.2 to 0.5 Pa·s and is a low-viscosity type of CMC-Na.
  • Neutral CMC-Na has a viscosity value of 0.3 to 0.6 Pa·s and 0.8 to 1.0 Pa·s and belongs to the medium-viscosity and high-viscosity types of CMC-Na.
• What is the difference between CMC and CMC-Na?
  CMC-Na is the sodium salt form of the CMC complex. The main difference between CMC-Na and CMC is that CMC-Na is easily soluble in both hot and cold water and is therefore easy to maintain, whereas CMC is easily soluble in water and is therefore difficult to maintain.

Advantages

• We provide fast CMC-Na analysis services through our efficient analysis process and professional team to help our clients get the data they need more quickly.
• We have accumulated rich experience in CMC-Na analysis and accurately interpret the analyzed data to provide more valuable information for our clients.
• We have established a complete Pharmaceutical and Biological Analysis system to help our clients analyze the physical, chemical, and biological properties of CMC-Na, and to provide a theoretical basis for revealing their biological activities and structure-activity relationships.

CD BioGlyco combines various technologies to help clients fully analyze CMC-Na and to help them better control the quality during production and R&D to ensure that their products meet the requirements. Please feel free to contact us if you are looking for a partner in CMC-Na research, we will be your best helper.