Profile of Biantenna Type N-glycans

Biantennary type N-glycan is a complex N-glycan that consists of two antennae, each of which can have a different sugar residue, such as galactose, fucose, and sialic acid. Biantenna-type N-glycans are widely found on proteins of eukaryotes and are involved in the processes of protein folding, stabilization, translocation, and function. Glycoengineering refers to a science that studies the structure, function, and metabolic regulation of the sugar chains of glycoconjugates and their applications. Applying the glycoengineering system to the analysis of antenna-type N-glycans is advantageous for the study of their functional applications.

Fig.1 The structure of biantennary N-glycan. (Alshanski, et al., 2020)

Cell Surface Biantennary Type N-glycan Glycoengineering Service at CD BioGlyco

At CD BioGlyco, we have accomplished systems in cell surface N-glycan glycoengineering services. We offer the following services in terms of glycoengineering services in cell surface N-glycans of the biantennary type:

• Structural analysis of biantenna type N-glycans.
  At CD BioGlyco, we utilize a comprehensive capability to analyze biantennary-type N-glycans glycosylation. Ion chromatography (LC) and LC-MS are also provided in combination to analyze and characterize this N-glycan. Finally, we will assign definitive glycan structures to each glycoform by performing glycan release and carbohydrate substructure analysis.
• Editing service on biantennary type N-glycans.
  We provide a two-step chemoenzymatic approach for selective editing of biantennary-type N-glycan isoforms on the surface of living cells. It consists of two steps: the first is a "deletion" step, which removes specific subclasses of heterodimeric N-glycans; and the second is an "insertion" step, which assembles explicit N-glycans back into pre-treated sugar sites. Such sugar-editing cells, carrying more homogeneous oligosaccharide structures, can enable a precise understanding of carbohydrate-mediated functions.

Fig.2 We offer services regarding biantennary type N-glycans. (CD BioGlyco)

Publication

• Paper Title: Selective N-glycan editing on living cell surfaces to probe glycoconjugate function.
• Technology: Selective editing of N-glycans
• Journal: Nature Chemical Biology
• Published: 2020
• Results: The authors utilized the substrate specificity of the ENGase family to efficiently distinguish N-glycans with different functions. The corresponding N-glycan isoforms were selectively converted from heterogeneous to homogeneous by two-step editing, "deletion" and "insertion". In addition, the application of this method in Lec4 CHO mutant cells realizes a "non-heterogeneous to homogeneous" glycan editing strategy, which will help to explore the precise glycan-related functions of membrane glycoproteins. As shown in Fig.3, the authors used a two-step strategy to achieve selective editing of core-focused and unfocused N-glycans on living cells. For core-focused N-glycan subclasses, heterogeneous glycan mixtures were selectively removed by a WT Endo-F3-catalyzed "deletion" step. Then, an azide-labeled salivary complex-type N-glycan oxazolidine (Az-SCT-ox, 1) was precisely assembled onto the Fucα1,6 GlcNAc portion of the cell surface, catalyzed by the Endo-F3 D165A mutant, to extend its biological function.

Fig,3 Cell-surface N-glycan editing in a subtype-selective manner by a two-step strategy. (Tang, et al., 2020)

Applications

• In the pharmaceutical industry, N-glycans can be applied to aid in the development of drugs.
• In the food processing industry, N-glycans can be used in research on the production of nutritional products with specific physiological functions for the human body.
• The N-glycan glycoengineering technology can be used to help scientists further investigate the structure and function of polysaccharides.

Advantages

• Every step of our process is quality-tested to ensure client satisfaction.
• We have a comprehensive glycoengineering platform that provides specific glycoengineering services on each type of N-glycan.

CD BioGlyco specializes in the construction of glycoengineered systems. We have the structure, function, and metabolic regulation of the glycan chains of glycans analysis services and their applications and have accumulated extensive experience. If you are interested in our services, please feel free to contact us.