Characteristics of Hybrid N-Glycans

Protein glycosylation is one of the most common post-translational modifications. Mammalian glycoproteins include three main types of glycan chains: N-linked glycan chains consisting of one or more monosaccharide units, O-linked glycan chains, and glycosylphosphatidylinositol (GPI) lipid anchors. Among other things, N-linked glycosylation refers to the adhesion of oligosaccharides to aspartic acid via nitrogen atoms. Hybrid glycans in N-glycans refer to those N-glycans that have both substituted mannose residues (linked to GlcNAc) and unsubstituted mannose residues. This type of glycan is an essential branch of glycomics, which plays an important role in biosynthesis and degradation in living organisms.

Cell Surface Hybrid Type N-glycan Glycoengineering Service at CD BioGlyco

A complete and comprehensive analysis of N-glycans in glycoproteins mainly includes the following aspects: N-glycan release and purification enrichment; N-glycan structure detection and analysis, etc. In many cases, derivatization of N-glycans will also be involved. At CD BioGlyco, we provide services such as structural analysis of hybrid N-glycans and functional applications.

• Hybrid N-glycan release, purification, and enrichment services.
  The release of glycan chains from glycoproteins is a necessary step in the shape analysis experiments of glycoprotein N-glycans. Among the methods for the cultural release of glycan chains are hydrazinolysis and β-elimination. In addition, desalination of glycans is an important step in the glycan analysis process. Methods for purification and enrichment of glycans include hydrophilic interaction chromatography (HIC), porous graphitic carbon (PGC), hydrazine chemical enrichment, and boric acid enrichment. Among them, hydrophilic chromatography and PGC enrichment are widely used because they are not selective for glycans.
• Hybrid N-glycans structural detection services.
  The multi-branched, linked, and non-template-driven enzymatic synthesis of glycans leads to complex and diverse structures, which increases the difficulty of glycan structure analysis and characterization. At CD BioGlyco, we use methods such as high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE) for the separation and analysis of N-glycans, and nuclear magnetic resonance (NMR) and mass spectrometry (MS) for the identification of glycan structures.

Fig.1 Service contents of the hybrid N-glycans we offer. (CD BioGlyco)

Applications

• Hybrid N-glycans can be used for the development of glycomics.
• Glycoengineering of hybrid N-glycans plays an important role in biomedicine. Structural analysis of N-glycans, derivatization, and other techniques can be used in drug production research.
• Glycan engineering techniques can be used to modify the structure of various glycans to expand the functionality of the glycans.

Advantages

• We have a powerful glycoengineering platform to provide custom glycoengineering services to our clients.
• We have a professional research team with extensive experience in N-glycan glycoengineering services.

CD BioGlyco has accumulated rich experience in glycan research. We provide purification, enrichment, structure detection, and metabolic regulation of hybrid N-glycans, and we hope to be the right hand of our clients in the glycan engineering technology of hybrid N-glycans. If you are interested in our services, please feel free to contact us.