Cell Wall Glycome Profiling
At present, more searches are focusing on the composition and structure of plant cell walls and the conversion rate of biofuels. CD BioGlyco has succeeded in cell wall glycome profiling in different cases. We have the confidence to be your essential research assistant in the field of glycobiology.
The structure and composition of plant cell walls are very different, depending on factors such as the species of origin, tissue type, developmental stage, and environment. depending on many factors, including the species of origin, tissue type, developmental stage and environment, etc. The changes in the physicochemical and/or ultrastructural characteristics of the cell wall largely depend on the relative composition and proportions of various cell wall components, including (but not limited to) cellulose, lignin, hemicellulose and pectin polysaccharides, and the interaction between these polymers.
Cell walls are inherently resistant to destruction by biological or chemical catalysts. This tenacious resistance is considered to be the main obstacle to the production of cost-effective cellulosic biofuels. Therefore, it is necessary to have a detailed understanding of the Therefore, to overcome the obstacle, it is necessary to have a detailed understanding of the plant cell wall structure.
In recent years, glycome profiling can be used to study plant cell wall on natural and pre-treated plant biomass samples and determine the relative abundance of matrix polysaccharides in plant biomass and the cell wall structure in different plant categories, thus enhancing the understanding of cell wall structure and composition.
Fig 1. Biofuel conversion.
Our glycome profiling service is built upon a robust and well-established immunological approach that leverages a comprehensive suite of tools for in-depth analysis.
The plant cell wall constitutes a topologically interlocked matrix of macromolecular polymers. Our technique deploys sequential solubilization via an escalating gradient of chemical perturbants—such as EDTA, sodium carbonate, and potassium hydroxide—to progressively disassemble this architecture. This tiered methodology facilitates the operational fractionation of the wall, permitting evaluation of the extractability and steric exposure of discrete glycan fractions. These isolates are subsequently subjected to high-throughput profiling, commonly via ELISA, to interrogate the presence of liberated glycan epitopes.
Traditional glycome profiling relies on qualitative interpretation of colored heatmaps, which can be subjective and make it difficult to detect subtle but significant differences. We have advanced this methodology by employing a mixed-model-based quantitative approach for data analysis. This method normalizes the data, accounting for the amount of material recovered in each extract, and provides statistically significant comparisons, moving beyond qualitative visual differences.
We offer a flexible and comprehensive glycome profiling service applicable to a wide array of plant materials, including but not limited to woody and herbaceous biomass, monocots, dicots, and specific tissues like roots, leaves, and stems. Our service is scalable for both small-scale academic research and large-scale industrial screening projects, providing solutions for:
Our comprehensive glycome profiling service follows a clear, multi-step process to ensure precise and reproducible results.
Your biomass or plant tissue sample is first prepared by removing non-structural components and grinding to a uniform particle size to ensure a consistent starting material for extraction.
The prepared material is subjected to a series of sequential extractions using different solvents. Each solvent targets and solubilizes a specific class of cell wall polysaccharides, providing a valuable insight into their association and integrity within the cell wall matrix.
The extracted fractions are then analyzed using our comprehensive panel of glycan-directed monoclonal antibodies in a high-throughput ELISA format. This step generates a unique "fingerprint" of the glycan epitopes present in each extract.
The raw ELISA data is processed using our advanced quantitative methodology. We generate detailed reports that include normalized values, statistical heatmaps, and a clear interpretation of the data, highlighting significant differences in the cell wall's glycomic profile.
DOI.: 10.1038/s41526-023-00312-0
Journal: npj Microgravity
Published: 2023
Results: In this study, the authors investigated how spaceflight affects cell wall composition in Arabidopsis thaliana roots using glycome profiling and immunohistochemistry. They analyzed root extracts from seedlings grown on the International Space Station (ISS) using the Vegetable Production System (Veggie) compared to Earth-grown controls. Glycome profiling with a diverse library of glycan-directed monoclonal antibodies revealed global increases in binding to specific non-cellulosic epitopes (xyloglucans, xylans, arabinogalactans) in space-grown roots. Immunohistochemistry on root sections confirmed these findings, showing more intense labeling for 10 antibodies targeting these epitopes in space samples. Notably, increased labeling of xylem-enriched unsubstituted xylan backbone epitopes suggested accelerated secondary cell wall formation in roots during spaceflight. This study demonstrates the utility of glycomics combined with immunohistochemistry for detecting microgravity-induced, tissue-specific changes in plant cell walls using limited biomass from space experiments.
Our cell wall glycome profiling provides comprehensive mapping of plant cell wall carbohydrate architectures and their interactions. We extend this expertise in complex glycan analysis to aquatic systems through our Marine Glycomics Service, featuring specialized marine glycan release protocols, advanced separation and purification techniques, customized derivatization approaches, and detailed structural characterization for complete biomolecular investigation of marine-derived carbohydrates.
CD BioGlyco has provided reliable and cost-effective plant carbohydrates research services to customers all over the world and has received unanimous praise. We will always keep full transparency in the experimental process and speed up the research of glycobiology for customers.
Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.
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