Chemical-based Oligonucleotide Synthesis Service

Synthesis Technology of Oligonucleotides

Oligonucleotide is a general term for a class of short-chain nucleotides in which multiple adjacent nucleotide residues are linked by phosphodiester bonds. It can be used in gene editing, DNA storage, gene synthesis, drug delivery, molecular diagnostics, nanomaterial science such as DNA origami, etc. They are mainly synthesized by biological and chemical methods. Among them, chemical methods are the most mature. The process is a multi-step sequential reaction. The yield of the target product is affected by the occurrence of side reactions (e.g., depurination, etc.) during the synthesis process. This also affects the efficiency of the purification later on. Therefore, the reaction process generally starts with the temporary protection of groups not involved in the reaction. After a round of condensation reaction, the protective groups on the protected groups are removed to form the target phosphodiester bond.

Chemical-based Oligonucleotide Synthesis Service at CD BioGlyco

CD BioGlyco is equipped with a professional research team and sophisticated laboratory equipment in oligonucleotides. Based on the different uses, we provide custom synthesis and Modification services with various specifications. We produce oligonucleotides by liquid-phase and solid-phase methods. Purification Methods are also improved.

Fig.1 Route of our solid-phase method. (CD BioGlyco)

The solid-phase synthesis method includes a 4-step cycle. Through the cycle of these 4 steps, the bases consistent with the sequence of the intended synthesized product are synthesized by extending them one by one in a 3’→5’ manner. In addition, we also produce various products by a liquid-phase chemical process.

The reaction efficiency of each round of chemical synthesis in the four-step method cannot be guaranteed to be 100%. The products obtained by this method are adulterated with products shorter than the target products sequence as well as other by-products of the chemical reaction. Therefore, the synthesized products usually need to be further purified to remove the chemical reaction by-products in the chemical reaction. We have also optimized our purification methods accordingly.

Through our advanced synthesis processes, we continue to expand the types and purity of oligonucleotides. We offer fluorescent probe primers, ultra-high purity oligonucleotides, long oligonucleotides, abbreviated primers, etc. In addition to the supply of small-size oligonucleotides, we also provide large-scale products.

Applications of Chemical-based Oligonucleotide Synthesis Service

• Oligonucleotides obtained by optimized synthetic methods can be used as molecular diagnostic primers for genetic diagnosis, detection of pathogenic microorganisms, etc.
• The factors affecting the physicochemical properties of oligonucleotides are mainly the chemical modifications. A variety of synthetic methods can meet the needs of different products with different physicochemical properties.
• Oligonucleotides are used in developmental studies for competitive inhibitors of transcription factors, nucleases, stimulators of the immune system, biotherapeutic agents for the treatment of diseases, etc.

Advantages of Us

• Our state-of-the-art laboratory facilities are equipped with a wide selection of synthesis methods and purification equipment. The optimized methods result in high product quality and low cytotoxicity.
• The influencing factors in the synthesis process are the length and quality of oligonucleotide. They are checked at any time during the synthesis process.
• We screen suitable carriers for solid-phase synthesis. The carrier is chemically stable. It also has some mechanical strength and good resistance to grinding and extrusion.

CD BioGlyco has a unique technological advantage in solid-phase and liquid-phase synthesis. We have short synthesis cycles and the ability to synthesize high-quality products quickly. Please feel free to contact us for more detailed information on oligonucleotide synthesis and price.