Chemoenzymatic Labeling (CL)-based Cell Surface Metabolic Oligosaccharide Engineering Service

Features of CL-based Cell Surface Metabolic Oligosaccharide Engineering (MOE)

Chemoenzymatic labeling (CL) is one of the powerful tools in bioengineering to incorporate cell surface marker molecules. In a chemical modification, molecules attach to the cell surface mainly through covalent bonds, hydrophobic interactions, and electrostatic interactions. Enzyme-based modifications have high specificity, rapidity, and mild conditions, and catalyze efficient reactions. Different kinds of peptidases, transferase, ligase, and oxidoreductase are the main uses of protein labeling. Combining chemical and enzymatic methods not only utilizes the advantages of green safety but also improves the efficiency of modification.

Fig.1 Systemic representation of CL labeling for cell surface modifications. (Kumari, et al., 2021)

CL-based Cell Surface MOE Service at CD BioGlyco

Combining the Glycoengineering Platform, CD BioGlyco provides high-quality CL-based cell surface MOE services under the operation of a professional team.

• Chemoenzymatic modification service

• Chemical based modifications
  CD BioGlyco has many years of successful experience in chemical modification. We provide a covalent attachment of chemical probes modification service, covalent conjugation modification service, electrostatic interaction modification service, and hydrophobic insertions service.
• Enzyme based modifications
  We have a wide variety of enzyme samples that can be used for enzymatic modifications. This mainly includes peptidases, transferases, ligases, and oxidoreductases.

Based on the above, our company provides CL service with high specificity. Combine the flexibility of the chemical method with the efficient catalysis of the enzymatic method to accelerate the project process of clients.

Fig.2 Schematic representation of CL-based cell surface MOE services. (CD BioGlyco)

Publication

Paper Title: Chemoenzymatic labeling of proteins for imaging in bacterial cells

Technology: Chemoenzymatic labeling

Journal: Journal of the American Chemical Society

IF: 15.0

Published: 2016

Results: For all four expressed cells, the band intensity was several times the background. The cells expressing CheA, and FtsZ were up to 6.6-fold and 6.7-fold. In cells labeled with 1, it was obvious that all the target proteins were fully modified, and no unmodified forms were found. The polar localization of the chemotactic protein and the septal localization of each cell division protein can be observed.

Fig.3 SDS-PAGE analysis and live-cell images for labeled protein. (Ho & Tirrell, 2021)

Applications

• Site-specific CL labeling as a tool to observe virus budding in real-time.
• CL labeling should prove broadly useful for protein imaging in bacteria.
• CL labeling has been used for different applications from protein cyclization to cell surface modification.

Advantages of Us

• Service full guidance tracking, each step is strictly controlled.
• A wide variety of enzymatic modifications are available for selection.
• Our technical support meets all the requirements of our clients.

CD BioGlyco is a professional company specializing in serving glycobiology. We provide high-quality Cell Surface Metabolic Oligosaccharide Engineering Services (MOE) to clients all over the world. We are capable of providing efficient and reliable services to accelerate our clients' scientific research by shortening the project time. Please feel free to contact us for further details.