Click Chemistry-based Cell Surface Chemical Conjugation Glycoengineering Service

What Is Click Chemistry?

Click chemistry belongs to a chemical reaction between pairs of reagents to exclusively react with each other under mild conditions and is effectively inert to naturally occurring functional groups such as the amine group. Click chemistry is mild conditions and high selectivity, therefore, it is widely used in bioconjugation, biolabeling, and material sciences in the pharmaceutical and biotech industry.

Fig.1 Schematic illustration of cell labeling via metabolic glycoengineering. (Takayama, et al.,2023)

Click Chemistry-based Cell Surface Chemical Conjugation Glycoengineering Service at CD BioGlyco

CD BioGlyco provides comprehensive strategies for click chemistry-based cell surface chemical conjugation glycoengineering service to our clients worldwide. We possess the most advanced equipment and unique R&D expertise to provide personalized service according to client's specific needs promptly. The chemical conjugation service offered by CD BioGlyco allows the generation of specific glycoconjugates with high affinity for both academic and industrial purposes.

• Cell surface glycan labeling and conjugation

We have extensive experience with various cell surface glycan labeling and can provide you with high-quality labeled reagents for your downstream applications. The most popular conjugations we can offer are shown below.

• Protein/Peptide conjugation
• Antibody conjugation
• Enzyme conjugation
• Oligonucleotide conjugation
• Protein labeling and conjugation

Labeled proteins help us to research specific molecular interactions in complex biological systems with high sensitivity.

• Azido-modified proteins and/or glycochains
• Alkyne-modified proteins and/or glycochains
• Copper (I)-catalyzed click labeling
• Copper-free click labeling
• Glycans imaging
• Based on the glycan labeling methods, strategies enabling cell-selective and tissue-specific glycan labeling and protein-specific glycan imaging have been developed.
• Analysis and characterization
• High-performance liquid chromatogram (HPLC)
• Mass spectrometry (MS)
• Gas chromatography-mass spectrometry (GC-MS)

Fig.2 Click chemistry-based cell surface chemical conjugation glycoengineering. (CD BioGlyco)

Applications

• The copper-free and less cytotoxic click chemistry reactions have allowed for the application of click chemistry to the field of medicine.
• Click chemistry is a tool for cell transplantation and drug delivery.
• It is widely used for the modification of biomolecules, such as nucleic acids, lipids, and proteins with various compounds.

Advantages

• Click chemistry is high yield, wide scope, less cytotoxic byproducts, high stereospecificity, and simple reaction.
• Click chemistry reactions process under physiological conditions and the producing chemical bonds are irreversible.
• A new type of copper-free click chemistry has brought about the successful application of click reactions to living cells without copper-induced cytotoxicity.

At CD BioGlyco, Cell Surface Chemical Conjugation Glycoengineering Services are processed under a strict quality control process to ensure client's complete satisfaction. Working with us will free up your time and remove uncertainty, helping you develop robust assays and obtain trustworthy data. If you are interested in our services, please feel free to contact us for more information.