Physiological Effects of Cytidine Triphosphate (CTP)

CTP is not only an important precursor of ribonucleic acid synthesis, but also a crucial energy material. Just like ATP, CTP is a high-energy molecule that acts as a coenzyme in glycerophospholipids and protein glycosylation. CTP blocks the activity of aspartate carbamoyltransferase, an enzyme involved in pyrimidine biosynthesis. In addition, it is a selective agonist for the P2X₁ purinergic receptor, which is essential for nervous system activity.

Fig.1 Chemical structure of CTP. (CD BioGlyco)

Synthetic Methods

Nucleic acid degradation uses 5'-phosphodiesterase to degrade RNA extracted from yeast, opening the way for the industrial production of nucleotides. The challenge lies in the complexity of the subsequent separation and purification, and the purity of the product cannot be guaranteed.

Using trichlorophosphate as a phosphate donor, the chemical synthesis method converts cytidine directly into CTP, and its yield can reach 90%. The enzymatic synthesis based on nucleoside triphosphate (NTP) cycle and regeneration has many advantages including mild conditions, fast reaction rate, and environmental friendliness. Many enzyme-catalyzed NTP regeneration strategies have been developed, including acetate kinase (ACKase), pyruvate kinase (PKase), polyphosphate kinase (PPKase), creatine kinase (CKase), etc. These reactions utilize different phosphate donors.

Fig.2 Chemical structures of different phosphate donors in NTP regeneration. (CD BioGlyco)

Cytidine Triphosphate (CTP) Production Service at CD BioGlyco

CD BioGlyco has extensive experience in CTP synthesis and has complete Platforms to help you obtain high-purity CTP products efficiently and cost-effectively. Our CTP products help keep your research in order.

• CTP in neurotransmission: CTP can affect the signal transmission of synapses, regulate neural activity, rebuild and repair nerve cells, restore the function of damaged nerve cells, and improve their anti-injury ability. CD BioGlyco provides you with high-quality CTP to support your application in the study of the nervous system as well as the development of therapeutic agents for neuropathy.
• CTP in luminescence analysis: CTP, as a phosphate donor of adenosine monophosphate (AMP), participates in the synthesis of adenosine triphosphate (AMP), allowing for its application in luminescence analysis.
• CTP in RNA/DNA synthesis: CTP is a precursor for large-scale in vitro synthesis of RNA. When ribose in CTP is stripped of an oxygen atom (dCTP), it can be used to synthesize DNA.
• CTP in extracorporeal transcription: Desired RNA fragments can be prepared by in vitro transcription using phage T7 RNA polymerase, and CTP is the raw material for this process.
• CTP in the development of antiviral therapy: CTP has the potential to facilitate the discovery of new antiviral therapeutics. 3'-Deoxy-3', 4'-dihydro-cytidine triphosphate (ddhCTP), a nucleotide analogue, has been reported to inhibit RNA polymerases in various viruses.

Applications

• Study on the improving effect of CTP on the nervous system and developing therapeutic agents.
• In vitro transcription.
• RNA/DNA synthesis.
• Development of antiviral nucleotide analogue therapeutic agent.

Highlights

• Mature chemical synthesis technology for CTP production.
• Enzymatic synthesis strategy based on NTP cycle and regeneration.
• Professional expert team.
• Affordable CTP production pipeline.

If you have a need for high-quality CTP products, our experts at CD BioGlyco will support your need with their extensive knowledge of CTP production. Please feel free to contact us for more details not only our CTP production service but also our NTP Production Service.