Cytisus scoparius Lectin (CSA) Production Service at CD BioGlyco

Lectins are glycoproteins with non-catalytic domains that are ubiquitous in plant species. It reversibly and specifically binds specific carbohydrates, such as mannose, galactose, N-acetylgalactosamine (GalNAc), N-acetylglucosamine (GlcNAc), sialic acid, etc., making it a useful tool for glycomic analysis. In addition, lectins also exhibit a wide range of biological activities in anti-tumor, antibacterial, and immunomodulatory aspects. Therefore, the purification and characterization of lectins have attracted extensive attention from researchers in the field of glycobiology. CD BioGlyco is a professional glycobiology company, providing one-stop Lectin Production Services for enterprises, universities, and scientific research institutes around the world. We produce lectins of plant, animal, and microbial origin using Purification Techniques such as ion exchange chromatography and affinity chromatography.

CSA is mainly derived from the seeds of the leguminous plant Scotch broom and specifically binds to GalNAc, especially the terminal β-GalNAc. CSA also recognizes terminal α-GalNAc epitopes of simple monosaccharides and disaccharides. Studies have found that this lectin prefers the LacdiNAc (GalNAcβ1-4GlcNAc) epitope, and its binding to terminal β-GalNAc is inhibited by sialylation or fucosylation at position 3 of the proximal residue. At CD BioGlyco, we provide clients with high-quality CSA extraction, purification, and characterization services to meet their needs. Our service process is as follows:

• Extraction and Purification of CSA

Ground legume Scotch broom seeds are extracted with a phosphate buffer solution at a suitable temperature for some time. The dialyzed extract is further fractionated by affinity chromatography to obtain purified lectin. The purified lectin extract is in liquid form and freeze-dried to convert it to a solid powder form.

• Characterization of CSA

Fig.1 CSA characterization service. (CD BioGlyco)

• Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)
  SDS-PAGE is an electrophoresis method that uses polyacrylamide gel as the supporting medium. On this support medium, the substances are separated according to their molecular size and molecular charge. It has an extremely high resolution, covering DNA fragments of 10-3,000 bp. We use SDS-PAGE to estimate the molecular mass of purified lectins.
• Assay of hemagglutinating activity
  Hemagglutination activity tests are often used to detect polyvalent molecules that recognize blood cells, looking for their sugar specificity. We test the specificity of the CSA by a hemagglutination inhibition assay and explore the effects of temperature and pH on the hemagglutination activity of this lectin.
• Fourier infrared spectroscopy
  Fourier infrared spectroscopy detects chemical bonds in molecules by generating infrared absorption spectra of solids, liquids, or gases, and has the characteristics of high sensitivity, high precision, and high resolution. We characterize CSA by Fourier infrared spectroscopy.
• X-ray diffraction
  X-ray diffraction is the most basic and important structural testing method, which is used for phase analysis, crystallinity determination, etc. We compare the peak pattern, intensity, width, size, and position of purified CSA with standard lectins by X-ray diffraction.

Publication

Advantages

• We provide a series of services from extraction, purification, and drying to characterization of CSA.
• The extracted lectins are purified by gel filtration chromatography, ion exchange chromatography, affinity chromatography, and hydrophobic chromatography.
• Fourier infrared spectroscopy has the advantages of high sensitivity, high precision, and high resolution to characterize chemical bonds in molecules.
• We provide our clients with a variety of CSA products to support your research.

CD BioGlyco not only provides clients with lectin production services from Natural Sources (plants, animals, and fungi) but also provides high-quality Recombinant Lectin Production services. If you happen to need to produce lectins, please do not hesitate to contact us and we will develop the best production proposal for you.