Metabolic reprogramming is a hallmark of cancer. Metabolites are not only intermediates in metabolic pathways, but also mediate epigenetic modifications, protein post-transcriptional modifications, and cell signaling. The intermediate metabolites of the tricarboxylic acid cycle can participate in various biological processes through various shuttle mechanisms. Glucose is decomposed into two molecules of pyruvate through the glycolytic pathway, and pyruvate is converted into acetyl CoA under the action of the pyruvate dehydrogenase complex. Acetyl-CoA then enters the TCA cycle. As a key part of glucose catabolism, the TCA cycle is strictly regulated. Therefore, the study of TCA cycle metabolites can provide new ideas for the study of glucose metabolic reprogramming in cancer. CD BioGlyco can provide quantitative analysis services of TCA cycle intermediate metabolites and functional analysis services of intermediate metabolites in cancer progression.

What We Do

• We provide quantitative analysis of TCA cycle metabolites based on metabolomics technology.
• We provide an analysis of tumor-promoting or tumor-suppressive effects of TCA cycle metabolites.

We provide the following TCA cycle metabolite analysis.

Service Case

To explore the effects of acute administration of TCA cycle metabolic intermediate X in vivo and in vitro models.

1. Screening of metabolically abnormal intermediate metabolite X in the TCA cycle of cancer cells by metabolomic analysis.
2. Cancer cells are treated with different concentrations of TCA circulating metabolite X at different times, and the effect of X on the proliferation of cancer cells is detected.
3. To test whether cancer cells can survive long-term in the medium in the presence of the TCA cycle metabolite X, and to screen for X-resistant cancer cell subsets.
4. Verify that the growth rate of cancer cells is strictly dependent on X.
5. To investigate whether X resistance modulates the signaling pathways of cancer cell survival, proliferation, and differentiation.
6. The morphological changes of X-resistant cancer cells and their morphological changes and distribution were studied by confocal laser.

Applications

• To explore the effects of TCA cycle intermediate metabolites on cancer immune activation and immunosuppression.
• To investigate the effect of exogenous TCA cycle metabolic intermediates on glucose metabolism in malignant tumors.
• To explore the relationship between TCA cycle metabolic intermediates and chemoresistance of cancer cells.

CD BioGlyco can provide quantitative detection services for TCA cycle metabolic intermediates and their functional analysis services in cancer progression. Our experienced teams of scientists, researchers, and technicians provide fast turnaround, high-quality services at competitive prices for worldwide customers. Our customers have direct access to our staff and prompt feedback on their inquiries. If you are interested in our services, please contact us for more details.