Difucosyllactose (DFL), as a fucosylated HMO, shows good biological activities. In this regard, CD BioGlyco has many years of scientific research experience and provides you with a high-quality service for DFL production.

Biological Activity of DFL

DFL is a tetrasaccharide, where a second L-fucose moiety has been added to the 3-position of D-glucose in 2'-Fucosyllactose (2'FL) via an α(1→3) bond. It has strong antibacterial activity against various pathogens, including group B streptococcus, which has been identified as a major cause of neonatal sepsis. A study evaluated the effects of three HMOs, 2'FL, 3-fucosyllactose (3-FL), and DFL, on the growth of probiotic and pathogenic bacteria. Bacterial groupings include Bifidobacteria, Lactobacillus, other human commensals, and potential pathogens. Most bacteria were found to grow well on commercial galacto-oligosaccharides, while DFL and 2'FL were the most selectively utilized carbohydrate sources, followed by 3FL. Compared with other prebiotics with lower selectivity, the selectivity of HMO can promote the growth of beneficial microorganisms but does not support the growth of harmful pathogens, thus facilitating the evolution of infant intestinal microbiota.

Fig.1 Effects of carbon sources on bifidobacterial growth. (Salli, et al., 2020)

New Food Ingredients Containing DFL

The reports on its biological activity and application are mostly mentioned together with 2'FL. Recently, a mixture expected to become a new type of food has attracted the attention of researchers. It is a powdery mixture mainly composed of two oligosaccharides, 2'FL and DFL (81% and 11%, respectively, average of five batches of the new type food) and other carbohydrates (e.g. D-lactose, L-fucose), 2'-L-fucosyl-D-lactulose), obtained by fermentation of transgenic Escherichia coli K12 strain using D-lactose and D-glucose as raw materials. Its production is intended to be used in infant food, food for special medical purposes, whole meal substitutes for weight control, food supplements, and various foods.

DFL Production Service at CD BioGlyco

CD BioGlyco supplies a variety of Methods that fully meet your production requirements for DFL. The optimization strategies we supply include but are not limited to the following:

• Enzymatic synthesis
• Using cheap substrate in a one-pot method such as D-lactose and D-glucose.
• Selection and identification of suitable enzymes.
• Optimal enzyme expression levels.
• Fermentation
• Construction of engineered strains.
• The fed-batch cultivation of the strains.
• Optimized separation and purification technology.
• Synergistically eliminating bottlenecks at thermodynamic and kinetic limits in synthetic pathways.

Advantages of Us

• One-pot synthesis.
• Low-cost production.
• Combined application of production technologies.
• Efficient and high-quality production.

With rich production experience on oligosaccharides, CD BioGlyco provides you with high-quality services for DFL production. If you have production needs about DFL, please contact us directly.

References:

1. Salli, K.; et al. Selective utilization of the human milk oligosaccharides 2'-Fucosyllactose, 3-Fucosyllactose, and difucosyllactose by various probiotic and pathogenic bacteria. Journal of Agricultural and Food Chemistry. 2020, 69(1): 170-182.
2. Turck, D.; et al. Safety of 2'-fucosyllactose/difucosyllactose mixture as a novel food pursuant to regulation (EU) 2015/2283. EFSA Journal. 2019, 17(6): e05717.