Disialyllacto-N-neotetraose (DSLNnT) is an important member of human milk oligosaccharides and has many potential applications. Based on years of experience in providing services in the field of glycobiology, CD BioGlyco provides professional and high-quality services for DSLNnT production.

Positive Effects as Derivatives of LNnT for DSLNnT

In recent decades, Human milk oligosaccharides (HMOs) have attracted the attention of scientists because of their rich physiological activities. As the basic component of HMO, two kinds of tetrasaccharides, namely lacto-N-tetraose (LNnT) and lacto-N-tetraose (LNT), each account for 6% (w/w) of the total HMO. These two oligosaccharides are also the core structures of sialylation and fucosylation and could generate longer chains of core structure through further derivatization and extension. LNnT, LNT, and their sialic derivatives and/or fucosylation account for more than 30% (w/w) of the total HMO. DSLNnT, as a derivative of LNnT, has been found by biologists to have a positive impact on anti-inflammatory and other aspects, and it has shown commercial value and application potential in the food and pharmaceutical industry.

Fig.1 Anti-inflammatory activity of DSLNnT. (Yu, et al., 2017)

Anti-inflammatory Activity of DSLNnT

Necrotizing enterocolitis is a very common gastrointestinal disease. Its occurrence is mainly related to many factors such as hypoxia and ischemia, bacterial or viral infection, and gastrointestinal immaturity. The mortality rate is as high as 10% to 30%, and the main patient group is premature infants. In the face of this highly vulnerable population, the corresponding clinical treatment methods are very limited. DSLNnT and DS'LNT have been proven to protect neonatal rats from necrotizing enterocolitis, which provides a new direction for clinical research on necrotizing enterocolitis in premature infants. Due to the limited availability of human milk, the corresponding content in milk is not enough to support the mass production of DSLNT. Biocatalytic Methods have become a more popular synthetic trend.

DSLNnT Production Service at CD BioGlyco

CD BioGlyco supplies a variety of Methods that fully meet the production requirements for DSLNnT. The optimization strategies we supply include but are not limited to the following:

• One-pot multienzyme reactions
  The production idea is to synthesize LNnT first and further synthesize DSLNT.
  Selection and identification of suitable enzymes including Escherichia coli galactokinase, glycosyltransferase, and pyrophosphorylase, etc.
  Comprehensive use of protein engineering, metabolic engineering, and synthetic biology strategies and tools.
• Microbial Fermentation
  Constructing engineered strains. The metabolic pathway is optimized through genome fine-tuning, overexpression, and other strategies.
• Combination of different production technologies, such as chemoenzymatic methods.

Advantages of Us

• Low-cost production.
• Response specificity.
• High efficiency and selectivity.
• Rich enzyme and bacterial resources.
• Green environmental protection.

CD BioGlyco is dedicated to providing one-stop service to help our clients achieve efficient production of LNnH. If you have production needs about DFL, please feel free to contact us.

Reference

1. Yu, H.; et al. Enzymatic and chemoenzymatic syntheses of disialyl glycans and their necrotizing enterocolitis preventing effects. The Journal of organic chemistry. 2017, 82(24): 13152-13160.