Embryo Implantation and Cultivation-based In Vivo Glycobiology Disease Model Development Service

Empowering Discovery Through Embryo Implantation: Your Gateway to Advanced Glycobiology Disease Modeling

CD BioGlyco offers a comprehensive range of professional services in the field of Glycobiology Disease Model Development. Our experienced senior professionals offer a highly advanced in vivo glycobiology disease model development service through cutting-edge Technologies and expertise, including embryo implantation and cultivation technology.

Firstly, CD BioGlyco selects appropriate animal models based on the specific purpose of the study and the characteristics of the disease of interest to ensure that the selected animal model mimics well the characteristics of the human disease. Afterward, we develop a comprehensive experimental protocol that encompasses the establishment of the disease model, processing of experimental animals, and specific observation time points. We take measures to ensure that our experimental design is logical and effectively captures the progression of the disease.
Our specialists acquire animal embryos by in vitro fertilization and embryo cultivation. Gene manipulation or insertion of particular genes is carried out during the cultivation process to replicate the initiation and progression of glycobiological disorders. The processed embryos are subsequently transferred into the uterus of the experimental animals with precision to ensure successful implantation and development.
We establish and develop the disease model by rigorously managing experimental animals and regularly monitoring their body weight, blood glucose levels, and other relevant physiological indicators. Disease phenotypes are observed and recorded in the animal models. Our experts observe and record the occurrence and development of disease phenotypes, collecting experimental data for statistical analysis to assess the validity and reliability of the model by comparing differences between the disease model group and the control group. Based on experimental results and experience, we optimize experimental protocols and technical processes to further improve methods of disease modeling for increased accuracy and reproducibility.

Process of embryo implantation and cultivation-based in vivo glycobiology disease model development service. (CD BioGlyco)

Publication

Technology: In-situ hybridization, Immunolabeling, Cell tracking, Transcriptome analysis

Journal: Nature Communications

IF: 17.694

Published: 2021

Results: This article discusses the use of experimentally designed signaling centers for morphogenetic factors to arrange stem cells to create models resembling mammalian embryos. The study discovered that when aggregates of mouse embryonic stem cells (ESC) were combined with these signaling centers, they formed structures similar to embryos, known as embryoid bodies. Through in-situ hybridization, immunolabeling, cell tracking, and transcriptome analysis, the researchers found that these embryoid bodies formed three embryonic layers through the process of embryonic germination and displayed extensive developmental structures similar to those of neural embryos. Ventrally, the embryoid body differentiates the primitive gut tube, which is patterned in both anterior-posterior and dorsoventral directions. Overall, the embryoid body provides an in vitro model of the mammalian embryo, demonstrates a wide range of germ layer derivatives development, and promises to be a powerful tool for in vitro studies and disease modeling.

Fig.1 Production of embryoids through the guidance of ESC aggregates with a morphogen signaling center. (Xu, et al., 2021)

Applications

The constructed disease models can be used to test the efficacy and safety of drugs, allowing for a faster and more accurate assessment of the potential effects of drug candidates.
The constructed models can be used to study and understand the pathogenesis of diseases in greater depth. This can help to reveal potential targets of disease and provide a basis for the development of new medical approaches.
These models can be used to study disorders of glucose metabolism, abnormal glycosylation, and other diseases such as diabetes and cancer.

Advantages

Our technology simulation of diseases in vivo provides a more accurate representation of their diversity and complexity.
Our technology in model development considers the physiological and biological variations among individuals, enhancing the reliability and relevance of the model.
The model constructed by our technology provides a more accurate and representative depiction of the disease within the human body as compared to in vitro cell culture techniques.

With a strong emphasis on quality, precision, and attention to detail, CD BioGlyco is committed to delivering reliable embryo implantation and cultivation-based in vivo glycobiology disease model development services that meet the highest standards in glycobiology research. If you are interested in further details, please feel free to contact us.

Reference

Xu, P.F.; et al. Construction of a mammalian embryo model from stem cells organized by a morphogen signalling centre. Nature Communications. 2021, 12(1): 3277.

This service is for Research Use Only, not intended for any clinical use.