In the field of Glycan Release, CD BioGlyco continuously updates the Enzymatic Release technology to ensure efficient and accurate glycan release services for clients. We have the confidence to be your best research assistant in the field of glycan release.
O-Glycosylation is a common post-translational modification of glycoproteins. Fundamentally different from N-glycosylation, the consensus amino acid sequence typical of O-glycosylation has not been clearly defined. Tyrosine (Tyr), hydroxylysine (hydroxy-Lys), or hydroxyproline (hydroxy-Pro) may be the peptide sites for O-linked glycosylation. Mucin-type glycans are the most common O-glycans and are involved in several important biological events, including intercellular communication in higher eukaryotes and adhesion between bacteria and host cells. Eight mucin-type core structures have been discovered. Core 1, also known as T antigen, is one of the most abundant core O-glycan structures. It links galactosyl β1,3-N-acetylgalactosamine (N-GalNAc) via an alpha bond to serine (Ser) or threonine (Thr) residue. Core 1 is also a precursor to more complex O-glycans such as extended core 1 and core 2 structures.
Fig.1 O-Glycan structures core 1-8. (Watson, et al., 2015)
O-Glycans are found on erythropoietin (EPO) or interleukin 2 (IL-2) and other human proteins such as immunoglobulin A (IgA) or mucin-1 (MUC1) also contain O-glycans sugar clusters. Since specific O-glycans are more abundant in tumors, it has been proposed to use peptides containing specific O-glycan structures as anticancer vaccines to elicit immune responses against cancer cell epitopes. O-Linked glycans attached to membrane glycoproteins or cell surface glycolipids are also involved in hematopoiesis and inflammatory responses. Core 1-derived O-glycans are involved in many important biological processes such as leukocyte lymphocyte homing, inflammation, and animal development.
O-Glycans are essential for physiological and pathological processes. A reliable method must be identified to better separate O-glycans from proteins before analysis. Conventional methods include enzymatic release methods and Chemical Release methods. At CD BioGlyco, we have developed an advanced glycan release platform. We provide our clients with efficient and accurate release services of core 1 O-glycans from native glycoproteins. We successfully cleave core 1 O-glycans and maintain protein structure by specific O-glycosidases. In addition, we provide clients with rapid and sensitive O-glycan quantification and characterization services.
Fig.2 The process of enzymatic release of core-1 O-glycan. (CD BioGlyco)
In terms of glycan release, CD BioGlyco is committed to improving the release efficiency and quality for clients to better quantify and characterize it. If you are interested in our core-1 O-glycan release service, please contact us for more information.
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