Enzymatic Release of Fucose
Fucose is released from fucose-containing polymers by an enzyme found in lysosomes called α-fucosidase. CD BioGlyco has advanced Enzymatic Release methods to provide clients with high-quality fucose release services.
Fucose is a deoxyhexose sugar with the chemical formula C6H12O5, which is present in a variety of glycoproteins and glycolipids produced by insects, plant cells, and mammals. In mammals, fucose-containing glycans play important roles in transfusion responses, host-microbe interactions, selectin-mediated leukocyte-endothelial adhesion, and many ontogenic events. Fucosylation is the transfer of fucose residues from guanosine diphosphate (GDP)-fucose donor to an acceptor molecule and is part of the ABO blood group and Lewis antigen systems. According to the different fucose addition sites, fucosylation is divided into α1-2-, α1-3/4-, α1-6-, and O-fucosylation. In humans, fucosylation is carried out by a group of 13 enzymes, each showing a specific activity.
The potential applications of fucose are becoming increasingly apparent in the fields of medicine and dietary supplements. In immunoglobulins, fucosylation of antibodies has been established to reduce binding to Fc receptors on natural killer cells, thereby reducing antibody-dependent cell-mediated cytotoxicity (ADCC). Therefore, fucosylated monoclonal antibodies have been designed to recruit the immune system to cancer cells and have been made in cell lines lacking the core fucosylase (FUT8), thereby enhancing cell killing in vivo.
CD BioGlyco leverages a suite of cutting-edge technologies to ensure precise and efficient enzymatic release of fucose. Our core technological platform integrates highly specific fucosidases, including alpha-L-fucosidases and beta-D-fucosidases, tailored for various linkage types (e.g., alpha1-2, alpha1-3, alpha1-4, alpha1-6). These enzymes are meticulously selected and optimized for purity and activity to ensure complete and selective fucose removal without compromising the integrity of the remaining glycan structure.
Enzymes are useful for the structural analysis of glycans and polysaccharides and for establishing structure-activity relationships in biological systems. Due to the biological importance of fucose, it is highly necessary to develop a systematic strategy to release fucose from protein. At CD BioGlyco, we have developed a Glycan Release platform to provide clients with efficient and accurate fucose release services. Fucosidase is a unique tool for hydrolyzing fucose on proteins. Under the action of fucosidase, we efficiently release α1-2, α1-3, and α1-4 linked fucose present on N-glycosylated proteins, O-glycosylated proteins, or free oligosaccharides.
Fig.1 The process of enzymatic release of fucose. (CD BioGlyco)
Our capabilities extend to various sample types, including purified glycoproteins, recombinant proteins, monoclonal antibodies (mAbs), serum, plasma, cell lysates, and tissue samples. We provide a comprehensive analysis of both N-linked and O-linked glycans, as well as glycosphingolipids, allowing for a holistic understanding of fucosylation patterns.
Journal: International Journal of Molecular Sciences
IF: 5.5
Published: 2024
Results: The article describes the identification and characterization of four new α-L-fucosidases discovered through a screen of an alpaca feces metagenomic library. The authors studied the enzymatic parameters of these fucosidases using p-nitrophenyl α-L-fucopyranoside as a substrate and explored their potential for use as transglycosidases with various mono-, di-, tri-, and oligosaccharides, as well as some amino acids. The findings highlight the hydrolytic capabilities of these novel enzymes and their promising applications in transfucosylation reactions, contributing to the broader field of enzyme discovery and glycoengineering.
CD BioGlyco not only provides a systematic experimental platform and advanced technical approaches, but also provides flexible guidance to help clients complete their scientific research work in a timely and efficient manner. If you are interested in our fucose release services, please contact us for more detailed information.
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