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Epilactose is a rare disaccharide formed by linking D-galactose and D-mannose through β-1,4 glycosidic bonds, which is an isomer with lactulose and lactose. Epilactose promotes the proliferation of Bifidobacteria and Lactobacillus in the intestinal tract of rats without causing an increase in blood sugar levels. It is a disaccharide with prebiotic properties, which is important for maintaining intestinal health and controlling intestinal diseases. Moreover, epilactose is not easily digested and absorbed by the intestinal tract of mammals, but it significantly increases the ability of the small intestine to absorb calcium and iron, reduces the level of plasma low-density lipoprotein and total cholesterol, and reduces the risk of atherosclerosis. Therefore, epifructose has broad application prospects.
Epilactose often needs to be isolated from complex mixtures (e.g. milk, syrups, biological fluids) containing many other sugars and carbohydrates. With the development of analytical technology, the test of epilactose has become more and more simple, fast, and accurate.
Fig.1 Epilactose analysis service. (CD BioGlyco)
Paper Title: Quantification of lactulose and epilactose in the presence of lactose in milk using a dual HPLC analysis
Technology: A dual HPLC analysis
Journal: Food Analytical Methods
IF: 3.498
Published: 2016
Results: The dual HPLC analysis (Fig.2) presented here was used for the precise test of epilactose, lactose, and lactulose in milk samples. After sample preparation, pre-column derivatization of carbohydrates was followed by dual HPLC analysis. A BDS Hypersil RP-C18 column combined with UV detection (Method 1) was used for the determination of epilactose and lactose content, as the sugar xylose can also be derivatized under the parameters chosen, it served as an internal standard for quantitative calculations. Using an isocratic solvent of 20 mM TBAHSO4 in sodium phosphate buffer is a mobile phase. The results (Fig.3) showed that the retention times of lactose, epilactose, and xylose were 14.5±0.1, 15.5±0.1, and 21.2±0.1 minutes, respectively. The methods all have good linearity, accuracy, and recovery rate.
Fig.2 Schematic diagram of the dual HPLC analysis for lactose, epilactose, and lactulose. (Rentschler, et al., 2016)
Fig.3 Chromatograms of various compounds in milk drinks. (Rentschler, et al., 2016)
CD BioGlyco will provide optimal epilactose analysis services for your samples and provide quick feedback on results. Please feel free to contact us promptly if you are interested in learning more about our analysis services.
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