Euonymus Europaeus Lectin Production Service

Euonymus europaeus Lectin (EEL) Production Service at CD BioGlyco

EEL is obtained from the fruit of the European spindle tree, which binds to blood group-related carbohydrates (mainly B and H determinants) and specifically recognizes terminal fucose-containing carbohydrates. CD BioGlyco has rich experience in Plant Lectin Production, and our professional technical team provides comprehensive EEL production service.

Plant tissue culture
- We use in vitro culture technology to achieve sustained and stable EEL production by cultivating plant tissues or cell lines, such as seeds, leaves, or roots.
- We extract and purify EEL from the collected plant tissues.
  - Cell disruption: We freeze or chop E. europaeus plant tissue and use extraction buffer to disrupt the cells and release EEL.
  - Solid-liquid separation: We separate solid cell residues and liquid extracts from the cell disruption fluid through centrifugation and other methods.
  - Protein precipitation: We add appropriate salt solutions (such as NaCl) or organic solvents (such as acidic ethanol) to the liquid extract to precipitate proteins, which helps remove impurities and some non-target proteins.
  - Chromatography: We use various chromatography techniques to isolate and purify EEL. For example, the specific sugar-binding ability of EEL is used to enrich EEL through sugar column chromatography or other sugar-binding affinity chromatography techniques. This method is very effective for rapid purification and enrichment of EEL.
- We perform activity assays on purified EEL and use glycosylation analysis, immunoassay, cell agglutination test, and other methods to detect and quantify the activity and content of EEL. These methods ensure that EEL products with expected activity are obtained.
- Finally, we process and store EEL products using concentration, freeze-drying, or other appropriate methods.
Multi-category EEL conjugation service

EEL is conjugated with a variety of compounds, such as alkaline phosphatase, biotin, rhodamine, etc. The purpose of conjugation is to introduce EEL into specific experiments or applications by combining it with specific compounds. CD BioGlyco utilizes the selective binding ability of EEL to specific sugar molecules to provide a variety of EEL Conjugation Products to detect, identify, or visualize biological processes, cellular structures, or disease markers related to specific sugar molecules, which helps to expand the application scope of EEL, making it a useful component in analytical tools, biomarkers, or drug development.

Fig.1 EEL production service. (CD BioGlyco)

Publication

Technology: Fluorescence titration, homology modeling

Journal: Glycobiology

IF: 5.954

Published: 2015

Results: The authors used EEL to perform fluorescence titrations on a range of carbohydrates, and the results showed that EEL had broad carbohydrate specificity. The authors used these data to guide homology modeling to investigate the structural basis for carbohydrate recognition by EEL. The results demonstrated that EEL had a β-trefoil fold structure and contained three potential carbohydrate-binding sites (α, β, and γ sites were shown in yellow, green, and pink, respectively), each site was contained in a single loop 20-30 amino acids in length, with binding sites typically starting with a leucine residue and ending with a tryptophan residue.

Fig.2 The folded structure of EEL. (Agostino, et al., 2015)

Applications

Immunohistochemistry: EEL binds to specific glycosyl structures in tissue samples and is used for immunohistochemical staining to observe and localize specific cell types and tissue structures.
Tumor marker detection: EEL combines with certain tumor-related glycosyl structures and is applied to the detection and evaluation of tumor markers, which is helpful for disease monitoring and research.
Tissue engineering: EEL is used as a bioadhesive agent for the combination of cell culture and tissue engineering scaffold materials in tissue engineering and regenerative medicine.

Advantages

Advanced technology: We have advanced production technology to ensure efficient EEL production and purification processes.
High-quality lectins: We provide high-purity, high-activity EEL products to ensure their reliability and effectiveness in applications.
Comprehensive analysis and reports: We respond quickly to client needs and provide comprehensive EEL product analysis and reports to help clients understand product properties and application potential.

CD BioGlyco actively invests in R&D and technological innovation to provide comprehensive Lectin Production solutions. We have sufficient production capacity and scale to provide high-level EEL production to meet different research needs. Please feel free to contact us if you need high-quality EEL production service.

Reference

Agostino, M.; et al. The carbohydrate-binding promiscuity of Euonymus europaeus lectin is predicted to involve a single binding site. Glycobiology. 2015, 25(1): 101-114.

This service is for Research Use Only, not intended for any clinical use.