Properties
Description
Fish lipopolysaccharide-binding protein (LBP) ELISA kit, an enzyme-linked immunosorbent assay, has the ability to detect levels of fish LBP as low as 1 ng/mL and its detection range is 10-320 ng/mL.
Assay Principle
Fish lipopolysaccharide-binding protein (LBP) ELISA kit is designed to measure the level of fish LBP in a sample. It utilizes purified fish LBP antibody to coat the microtiter plate wells, creating a solid-phase antibody. The sample is then added to the wells, allowing the fish LBP to bind to the solid-phase antibody. HRP-labeled antibodies specific to fish LBP are subsequently added, forming an antibody-antigen-enzyme-antibody complex. After thorough washing, a TMB substrate solution is added, resulting in a blue color development due to the catalytic activity of the HRP enzyme. The reaction is stopped by adding a sulfuric acid solution, and the color change is measured at a wavelength of 450 nm using a spectrophotometer. The concentration of fish LBP in the samples is determined by comparing the absorbance (O.D.) of the samples to a standard curve.
ELISA Type
Sandwich ELISA kit
Assay Type
Sandwich ELISA kit
Components
Closure plate membrane; Sealed bags; Micro ELISA stripplate; Standard; HRP-Conjugate reagent; Sample diluent; Chromogen solution A; Chromogen solution B; Stop solution; Wash solution
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Method
Colorimetric, OD 450 nm
Precision (Intra-Assay)
CV <10%
Precision (Inter-Assay)
CV <15%
Applications
Fish lipopolysaccharide-binding protein (LBP) ELISA kit can be used to measure the level of fish LBP in serum, plasma, tissue homogenate and other biological fluid samples.
