Properties
Description
Fish lipopolysaccharide (LPS) ELISA kit, an enzyme-linked immunosorbent assay, has the ability to detect levels of fish LPS as low as 0.1 ng/m and its detection range is 2-64 ng/mL.
Assay Principle
Fish lipopolysaccharide (LPS) ELISA kit is designed to measure the level of fish LPS in a sample. It utilizes purified fish LPS antibody to coat the microtiter plate wells, creating a solid-phase antibody. The sample is then added to the wells, allowing the fish LPS to bind to the solid-phase antibody. HRP-labeled antibodies specific to fish LPS are subsequently added, forming an antibody-antigen-enzyme-antibody complex. After thorough washing, a TMB substrate solution is added, resulting in a blue color development due to the catalytic activity of the HRP enzyme. The reaction is stopped by adding a sulfuric acid solution, and the color change is measured at a wavelength of 450 nm using a spectrophotometer. The concentration of fish LPS in the samples is determined by comparing the absorbance (O.D.) of the samples to a standard curve.
ELISA Type
Sandwich ELISA kit
Assay Type
Sandwich ELISA kit
Components
Closure plate membrane; Sealed bags; Micro ELISA stripplate; Standard; HRP-Conjugate reagent; Sample diluent; Chromogen solution A; Chromogen solution B; Stop solution; Wash solution
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Method
Colorimetric, OD 450 nm
Precision (Intra-Assay)
CV <10%
Precision (Inter-Assay)
CV <15%
Applications
Fish lipopolysaccharide (LPS) ELISA kit can be used to measure the level of fish LPS in serum, plasma, tissue homogenate and other biological fluid samples.
