Properties
Description
Oligo-α-1,6-glucosidase from microbial is responsible for hydrolyzing α-1,6-glucoside bonds from the non-reducing ends of isomaltooligosaccharides and palatinose. β-Galactosidase from Aspergillus niger, a crucial enzyme in the human body, is responsible for hydrolyzing galactosides into galactose.
Molecular Weight
67.3 kDa; 125 kDa
Unit Definition
Oligo-α-1,6-glucosidase from microbial: One unit of oligo-α-1,6-glucosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-α-D-glucopyranoside (5 mM) in sodium acetate buffer (50 mM), pH 4.5 at 40°C.
β-galactosidase from Aspergillus niger: One unit of β-galactosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-β-D-galactoside (10 mM) in sodium acetate buffer (100 mM), pH 4.5 at 40°C.
Source
Oligo-α-1,6-glucosidase: microbial; β-galactosidase: Aspergillus niger
Applications
This product is applied to measure isomaltose and lactose in dietary fiber.
