Properties
Description
β-Galactosidase from Aspergillus niger, a crucial enzyme in the human body, is responsible for hydrolyzing galactosides into galactose.
Unit Definition
One unit of β-galactosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-β-D-galactoside (10 mM) in sodium acetate buffer (100 mM), pH 4.5 at 40°C.
Applications
β-Galactosidase from Aspergillus niger is applied for the structural research of sugar, the measurement of lactose, and as an enzyme label for enzyme immunoassay.
