Description
The elution buffer is used for eluting bound glycoproteins from lectins DSA/DSL, GSL II, LEL/LEA, WGA, Succ-WGA and UDA. It helps maximize the yield of lectin DSA/DSL, GSL II, LEL/LEA, WGA, Succ-WGA or UDA, along with preserving the lectins activity for repeated use. Chelating agents are used to react with metal ions in order to form a stable complex. This solution contains 2 mM of chelating agent EDTA, 0.1 M of GluNAc, and 1 M of NaCl. The solutions pH is in neutral range and the eluting components can be removed by dialysis.