Glucosamine Analysis Service

Biological Activity of Glucosamine

Glucosamine is a compound obtained by replacing a hydroxyl group in the glucose molecule with an amino group. It is widely found in animal cartilage and crustacean shells. Glucosamine can promote the synthesis of cartilage and inhibit its breakdown, with the ability to combat the damage done by inflammation and stimulate the growth of cartilage cells. Glucosamine can also protect lipid macromolecules from oxidative damage by hydroxyl radicals, showing strong antioxidant activity. In addition, it has the function of stimulating the immune response and enhancing the body's immunity.

Fig.1 Chemical structure of Glucosamine. (Wikipedia)

Glucosamine Analysis Service at CD BioGlyco

CD BioGlyco provides clients with a one-stop glucosamine metabolism analysis service. Our core analysis process is shown as follows.

Fig.2 Flowchart of glucosamine metabolism analysis. (CD BioGlyco)

• Preparation of solutions
  
  • Solid sample: The solid is accurately weighed, fully dissolved in trifluoroacetic acid (TFA) solution, and then blow-dried with nitrogen gas. Repeated cleaning with methanol is performed several times. Finally, the sample is dissolved in sterile water and put into a clean chromatographic bottle.
  • Liquid sample: The liquid is concentrated or blow-dried with nitrogen gas into a solid. The subsequent steps are consistent with the preparation of the solid sample.
  • Standard solution: The glucosamine powder is accurately weighed and fully dissolved with sterile water to prepare a control solution.
• Instrumental analysis: Ion chromatography system and liquid chromatography column are used. The injection volume is 5 μL. Mobile phase A is H₂O, and the NaOH solution works as mobile phase B. The glucosamine component is detected and analyzed by the electrochemical detector.
• Qualitative and quantitative analysis: By high-performance liquid chromatography (HPLC) separation, the glucosamine component is qualitatively analyzed based on the retention time of the standard solution and components in the sample. The concentration-peak area curve is drawn by testing standard solution, and the concentration of glucosamine in the sample is calculated according to the peak area.
• Quality control: CD BioGlyco implements strict quality control over the whole process of detection and analysis. We detect the sample repeatedly to ensure the stability and reliability of the results.
• Technical report: CD BioGlyco generates detailed data reports, including experimental procedures, parameter settings, spectrograms, raw data, and final summary. If you need other content to be included, please tell us your requirements. Generally, you will receive our technical report 3~5 weeks after the sample is sent.

Applications

• Qualitative and quantitative detection of glucosamine
• Analysis of glucosamine content in complex samples
• Study on glucosamine metabolic pathway
• Study on anti-inflammatory and antioxidant activity of glucosamine

Highlights

• Efficient HPLC detection and analysis solutions
• The capacity to accurately analyze glucosamine in complex samples
• Quality control of the whole process
• Excellent 24-hour after-sales service

CD BioGlyco is committed to providing complete services to support scientific research. Our Carbohydrate Metabolism Analysis services are well received by clients around the world. If you are looking for personalized Solutions, we are your best choice. Please contact us for more information.