Glucose tolerance test can be divided into oral glucose tolerance test and intraperitoneal glucose tolerance test. It has been reported that dysregulation of glucose metabolism may be involved in the occurrence of cancer. Research on the relationship between glucose tolerance and cancer risk has found that a rapid drop in plasma glucose can increase cancer risk. Therefore, the glucose tolerance test contributes to a better understanding of the mechanism by which glucose metabolism regulates cancer development. CD BioGlyco provides efficient glucose tolerance testing services and real and effective experimental data.

Fig. 1 Glucose tolerance tests profile (Benedé-Ubieto R, et al., 2020)

Establishment of Impaired Glucose Tolerance Mouse Model

We are able to establish a high-fat diet-induced mouse model of impaired glucose tolerance, the specific steps are as follows:

8-week-old mice are selected and fed with a high-fat diet for 2-3 weeks, and their blood glucose values at each time point are detected by glucose tolerance tests.

Our Experimental Protocol

Fig. 2 The protocol of glucose tolerance tests

Our Features

• The experimental procedure strictly controls the effects of other variables on the mice. For example, pre-warming the glucose solution before injecting it avoids discomfort to the mice caused by the injection of a cold solution.
• During the experimental operation, the guidelines for the ethics of laboratory animals and the guidelines for the economics of laboratory animals were strictly followed. For example, when tail vein blood sampling is performed, no more than 2 mm of tail tissue is removed.

Applications

• To study the difference in glucose tolerance between tumor-bearing mice and normal mice, and analyze the effect of glucose tolerance on tumor growth.
• To study the effect of glucose metabolism regulators on glucose tolerance in tumor-bearing mice.
• To explore the regulatory network between glucose tolerance and tumorigenesis.

CD BioGlyco provides efficient glucose tolerance testing services and real and effective experimental data. Our experienced teams of scientists, researchers, and technicians provide fast turnaround, high-quality services at competitive prices for worldwide customers. Our customers have direct access to our staff and prompt feedback on their inquiries. If you are interested in our services, please contact us for more details.

Reference:

1. Benedé-Ubieto R, et al. Guidelines and Considerations for Metabolic Tolerance Tests in Mice. Diabetes Metab Syndr Obes. 2020 Feb 18; 13: 439-450.