Unlocking the Power of Glycans: Expertly Tailored Cells for Advanced Biomedical Solutions

Cell-based glycan arrays can predict the structural features of glycans and can be applied in different ways to a wide range of fields. CD BioGlyco has constructed Glycan Display Platforms to develop different types of glycan display arrays. Among them, Glyco-engineered Cell Construction is an important element in the development of cell-based glycan display arrays. For this reason, we offer a glycan-engineered cell construction service based on the glycan capping pathway.

• Glycan array strategy
  First, we determine the different glycosylation pathways by integrating and analyzing the organism's glycosyltransferases.
  Next, the genes for glycosyltransferases are assigned among the glycosylation pathways with different pathway functions, which are used to predict the structure and characteristics of glycans. Among them, some genes are classified as pathway non-specific caps, including sialylation and fucosylation.
  Then, we set up the HEK293 cell line as a platform for the construction of the glycan capping pathway. We analyze glycosyltransferase genes and their proposed functions using RNA-sequencing (RNA-seq) transcriptomics to predict the glycosylation capacity of the cells, as well as pathway-specific capping features.
• Glycan sublibrary-based construction
  At first, we identify the binding proteins of the glycan capping pathway, as well as the glycosyltransferase genes involved. Next, glycan epitopes are analyzed.
  • If the epitope of the glycan is known, bind it to the library containing the glycosylation feature.
  • If glycan epitopes are partially known, select a sublibrary bound to the knockout or knockin epitope of the pathway-specific gene associated with the glycan epitope.
  • The beginning library shall be chosen if the epitope is not known.
  After binding research with the corresponding sub-libraries, the glycosyltransferase genes required for binding are identified. Further sublibraries are selected based on the identified genes and their predicted functions, and cell construction is completed by linking data from different sublibrary screens.

Fig.1 Glycan capping pathway-based glyco-engineered cell construction. (CD BioGlyco)

Publication

Applications

• Glyco-engineered cell constructs based on the glycan capping pathway can be used to research recombinant expression and characterization of glycoproteins.
• Glyco-engineered cell constructs based on the glycan capping pathway can be used to expand and advance cell-based glycan arrays.
• Glyco-engineered cell constructs based on the glycan capping pathway can be used to predict the structural characteristics of related glycans.

Advantages

• We offer our clients multiple routes to glycan-engineered cell constructs to ensure that their requirements are met.
• Our technology is world-leading and suitable for constructing various types of glycan display arrays.
• We provide comprehensive pre-sales and after-sales services, as well as detailed report interpretation.

Relying on state-of-the-art equipment and technology, CD BioGlyco provides high-quality, custom cell-based glycan display array construction services to clients all over the world. All of our protocols and strategies have been developed by scientists in the field of glycobiology and have passed quality tests to ensure that they meet the scientific needs of our clients. If you are interested in our services, please feel free to contact us.