The role of glycolipids is to maintain membrane stability and promote cell recognition and there are more and more researches on glycolipids. CD BioGlyco provides advanced glycolipid profiling platforms and powerful data analysis tools, and we have confidence to be your essential research assistant in the field of glycobiology.

Background

Whether prokaryotic or eukaryotic, each cell is surrounded by a thin amphiphilic semi-molecule permeable membrane layer called the plasma membrane, which can be used as a cell barrier between the internal and external environments. The main component of the plasma membrane is glycolipid (GL), which contains a hydrophobic lipid tail (localized in the cell membrane) and a hydrophilic sugar head in the external environment, thereby promoting the interaction with molecules dissolved in the surrounding environment. According to the structure of the lipid moiety (which may be diacylglycerol, polyisoprene, sterol or ceramide), GL can be categorized into glyceroglycerolipids, glycosylphosphatidylinositol and glycosphingolipids (GSL).

Glycolipids are conjugated macromolecules, which participate in cell-to-cell communication and exhibit important biological functions for various life processes, such as cell growth, differentiation, adhesion, proliferation, aggregation and dissociation, regulation of signal transduction, neurodegenerative and carcinogenic transformation. The biological specificity of glycolipids is mainly attributed to the carbohydrate structure. The amphiphilic nature of glycolipids means that a single extraction scheme is impractical for all different molecular species, and special techniques are required to distinguish neutral glycolipids from acid glycolipids (gangliosides).

Fig 1. The fluid mosaic model of the plasma membrane (Sarbu, M.; Zamfir, A.D. 2018)

Services

CD BioGlyco provides comprehensive glycolipid profiling services, and the technology platforms we use including but not limited to:

• Determination of carbohydrate and lipid profiles and linkage analysis of the two types of components mainly use chemical methods combined with gas chromatography (GC), HPLC, TLC and mass spectroscopy (MS).
• Enzymatic methods can be used to determine the linkage sequence and anomeric configuration of the oligosaccharide structure, and the same parameters can be determined with increasing frequency by immunological methods. Enzymatic method can also be used for linkage analysis of fatty acids in diacylglycerol species.
• Depending on the type of glycolipids, we will use matrix-assisted laser desorption/ionization (MALDI), tandem mass spectrometry (MS/MS), or fast atom bombardment (FAB).

Applications

• Cell-cell interactions and signal transduction research
• Immune system research
• Function and structure research of glycolipids
• Pharmaceutical and biomedical research

Advantages of Us

• Advanced and multiple technology platforms
• Experienced researchers
• High accuracy and consistency
• One-stop service

CD BioGlyco has quite a wealth of experimental experience in glycolipid profiling. Our efficient work and thoughtful service attitude will bring convenience to customers' research.

Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.

Reference:

1. Sarbu, M.; Zamfir, A.D. Modern separation techniques coupled to high performance mass spectrometry for glycolipid analysis. Electrophoresis. 2018, 39.