Overview

Glyconanoparticles exhibit multifaceted effects and hold significant promise in drug delivery and bioimaging. They integrate the benefits of a nano drug delivery system with the multivalent interactions of sugars, thereby significantly enhancing drug targeting capabilities. Assessing their cytotoxic effects is crucial for researchers to evaluate their safety and effectiveness in biomedical applications. CD BioGlyco has a well-established GlycoNano™ Platform and offers a comprehensive glyconanoparticle cytotoxicity assay service.

Empowering Glyconanoparticle Cytotoxicity Assay Through Cutting-edge Technologies

We have established a comprehensive Preclinical Study system for glyconanoparticles to help advance its use in biomedical applications. The entire program is executed through the following workflow to comprehensively assess the in vitro cytotoxicity of glyconanoparticle:

Glyconanoparticle Development

We are well-versed in various glyconanoparticle preparation methods and develop fucose, lactose, and galactose residue-modified glyconanoparticles, among others. Then we separate glyconanoparticles from unreacted substances and excess reagents by size exclusion chromatography, dialysis, etc., for purification. The products are characterized in detail by nuclear magnetic resonance (NMR), dynamic light scattering (DLS), scanning electron microscopy (SEM), and other technologies.

Cytotoxicity Assay

We assessed the cytotoxic effects of glyconanoparticles on different cell lines, including cell viability, proliferation, and morphology after exposure to glyconanoparticles. Appropriate controls, concentrations, and exposure times are selected based on various study objectives. Specific processes include the following:

• Cell line culture
  Cancer and normal cells expressing target antigens are cultured for glyconanoparticle cytotoxicity analysis. We have experience with virtually all types of culture formats and meet a wide range of experimental needs.
  • Cancer cell lines (e.g. HeLa, MCF-7, A549)
  • Primary human cells (e.g. HUVECs, HMSCs)
  • Other cell types
• Detection
  Cytotoxicity assays measure changes in a range of indicators including mitochondrial function, cell proliferation, cell viability, phospholipid deposition/lipid denaturation, DNA damage, and cell cycle. Through a series of assays, we determine the optimal exposure time (e.g. 24, 48, or 72 hours). Positive and negative controls are set up throughout the process to ensure the reliability of the assay.

  MTT assay	The method measures cell viability based on metabolic activity.
  Flow cytometry	It provides a rapid and reliable method to quantify viable cells in cell suspension.
  lactatedehydrogenase (LDH) assay	This method is used to detect cell membrane integrity and cytotoxicity.
  XTT assay	The method is used to assay mitochondrial dehydrogenase activity in living cells.
  Annexin V/PI staining	This method is one of the most sensitive, efficient, and proven methods for the detection of apoptotic cells.
  Morphological observations	It uses microscopy to detect apoptosis by morphology.

Data Analysis

The percentage of cell viability, etc., is calculated based on experimental data. We compile and analyze the data collected from these analyses using appropriate software packages.

Experimental Delivery

• Detailed description of the experimental methodology
• A synthesis of results and conclusions.
• Graphical representation of data (e.g., cell viability chat)

Workflow

We offer a one-stop glyconanoparticle cytotoxicity assay service. This process includes customized experimental design to meet your specific research needs and comprehensive interpretation of results. We will also discuss the results with you to gain insights and understand the implications of your study.

Applications

• The glyconanoparticle cytotoxicity assay is essential for the development of safe and efficient targeted drug delivery systems.
• The glyconanoparticle cytotoxicity assay is used to explore safer and more effective nanomedicines and therapeutics.
• The glyconanoparticle cytotoxicity assay is essential for the development of biosensors for the detection of various biomolecules.

Advantages

• We provide pre-sales consulting services to help you explore glyconanoparticles and design related experiments efficiently.
• Our experienced researchers customize the experimental process according to different experimental requirements and provide excellent after-sales service.
• Strict quality control processes, including validation of assays, positive and negative controls, etc., ensure reliable and reproducible results.

Publication Data

Frequently Asked Questions

• What cell types are used in the cytotoxicity assay?
  We have a variety of cell lines for the glyconanoparticle cytotoxicity assay, including, but not limited to, cancer cell lines, primary cells, and immortalized cell lines. Common cell choices include HeLa, MCF-7, NIH 3T3, etc. Please communicate with us for detailed analysis requirements.
• What kind of results do I get from a cytotoxicity assay?
  Our cytotoxicity assay reports include comprehensive data on cell viability, dose-response curves, and cytotoxic effects. In addition, the assay report contains statistical analysis and interpretation of results to assist you in your glyconanoparticle preclinical studies.
• How do I submit samples for testing?
  After communicating about a specific glyconanoparticle cytotoxicity assay project, we will provide you with a detailed sample submission protocol, including shipping instructions and the necessary documentation to guide the sample collection, storage, and shipment according to our specifications for optimal results.

CD BioGlyco provides a high-quality glyconanoparticle cytotoxicity assay service to help explore its safety and application in therapeutic and diagnostic approaches. If you are interested in our glyconanoparticle cytotoxicity assay service, please feel free to contact us with specific information, such as the type of cells you are interested in or any other special requirements, to develop a solution. We look forward to assisting you in a wide range of glyconanoparticle research endeavors!