To satisfy our customers' scientific needs in exploring the structures of glycosphingolipids (GSLs), CD BioGlyco's dedicated research teams have developed GSLs microarray analysis techniques to help customers analyze multiple samples simultaneously and provide valuable glycan-binding information. Our efficient and professional services will help our clients accelerate their research progress.

Introduction

GSLs are ubiquitous glycoconjugates found on cell membranes and they have important functions in many biological processes such as signal transduction, cell adhesion, embryonic development, and carcinogenesis. Understanding the role and immunological interactions of abnormally expressed glycolipids may be critical for the development of therapeutic agents for cancer and neurological diseases. However, identifying unknown GSL-glycan structures remains a major challenge.

Over the past few years, developments in MS-based technologies have offered valuable insights into the structural analysis of GSLs through the combination of efficient separation techniques and high-resolution MS. However, the complexities of biosynthesis, structure, and function of GSL increase the difficulty of GSL structural analysis. Currently, the advent of lectin-related high-throughput glycomics technologies (e.g. lectin microarrays) has made it possible to explore multiple different binding interactions simultaneously, providing an effective tool to detect and reveal the glycan patterns of GSL extracts.

Fig.1 Flow chart of the comprehensive analysis of GSL-glycans by lectin microarrays and MALDI-TOF MS and glycopatterns of GSL-glycans from liver cell lines. (Du, 2019)

Our Services

To identify unknown GSL-glycan structures, CD BioGlyco has developed various GSL microarray analysis strategies especially based on lectins to provide customers with sensitive and high-throughput glycan-binding information for cells, antibodies, or other proteins of interest. Techniques we provide include but are not limited to:

• We provide a comprehensive analysis of GSL glycans through a combination of lectin microarrays and MALDI-TOF mass spectrometry.
• We have established a new strategy for lectin microarray-based analysis of GSL-glycans in cultured cells that directly detects and reveals the glycan pattern of GSL extracts without releasing the glycans. The procedure includes:

Application

• Identification of exact structures of GSL-glycans
• Understanding of GSL-related diseases
• Providing information for diagnosis and treatment

Advantages of Us

• Analysis with high sensitivity and high-throughput
• A number of samples can be detected simultaneously and reliable results can be obtained
• Practical and simple methods
• Detailed after-sales service

CD BioGlyco is committed to technological innovation and development in the field of glycobiology. Our GSL microarray analysis service allows for simultaneous large-scale analysis, providing glycan patterns and detailed linkage information of GSL-glycans. Please contact us directly if you need technical support, we will try our best to provide you with the best solution.

Reference:

1. Du, H.; et al. Analysis of glycosphingolipid glycans by lectin microarrays. Analytical Chemistry. 2019, 91(16):10663-10671.