Glycosylation

Most membrane proteins are proteins with N-glycosylation or O-glycosylation modifications. Glycans have no specific secondary structure and have very strong mobility, which can shield most areas of the protein surface. The high degree of freedom of glycan conformation hinders the elucidation of the overall glycoprotein structure. Glycoprotein structure can be analyzed by computer simulation, but it needs […]

Glycosylation is a common tool in drug development. The introduction of sugar units can often change the water solubility, targeting, metabolic stability and other properties of the target molecule. However, the chemical method of introducing sugar units into the target molecule still faces many challenges. Most glycosylation reactions go through oxonium intermediates or their equivalents, […]

On June 17, 2024, the team of Dawen Niu from Sichuan University and Academician Yundong Wu from Peking University published a research paper titled "Catalytic Glycosylation for Minimally Protected Donors and Acceptors" in Nature. The study reports a Glycosylation platform that enables selective coupling between unprotected or minimally protected donor sugars and acceptor sugars to produce […]

Cell membrane glycoproteins are often highly fucosylated and sialylated, playing important roles in protein signaling, binding, and cellular processing functions. However, due to technical limitations, it is difficult to characterize and observe fucose-mediated protein-protein interactions. Carlito B. Lebrilla's team from UC Davis published an article titled "Protein oxidation of fucose environments (POFE) reveals fucose–protein interactions" in […]

In our general understanding, RNA always functions in the nucleus or cytoplasm. Even if it appears on the cell membrane, it is considered to have only weak and unstable interactions with the cell membrane. However, in 2020, researchers found that RNA does exist on the cell membrane of human monocytes, and the corresponding Oligonucleotide Fragments antagonized […]

Glycosylation is a key protein post-translational modification that affects protein folding, stability, activity and transport, and is an important quality attribute of protein drugs. A common glycoengineering method is to genetically modify the host cell's native glycosylation pathways, but this method is time-consuming and may interfere with normal cell growth and lead to a heterogeneous glycoprofile. […]