Glycosphingolipids (GSLs) play critical biological roles in cells, including the regulation of processes such as signal transduction, cell proliferation, and apoptosis, in addition to being implicated in the development of several diseases. CD BioGlyco provides GSLs component analysis services to our customers by enzyme-linked immunosorbent assay (ELISA).
ELISA is a biomolecular detection technique widely used in biochemistry, medicine, and other fields for the detection of specific proteins, antibodies, cells, etc. ELISA technology uses specific antibodies to bind to the target biomolecules and achieves the detection, quantification, and characterization of the molecule to be tested through the technique of enzyme labeling. Its main principle is to use the specific binding effect between the fixed antigen, antibody, and the substance to be detected, and after the reaction and washing, the substance is finally detected and quantified by the enzyme marker. It is widely used in the fields of medicine, biochemistry, molecular biology, and environmental science because of its rapid and accurate detection of a wide range of substances such as cytokines, viruses, bacteria, tumor markers, and antibodies. It has now become one of the indispensable assays in the field of biochemistry and medicine.
Fig.1 Direct ELISA protocol. (Shah,et al., 2016)
CD BioGlyco has developed an efficient and reliable ELISA service package to help our customers explore the close relationship between GSL components and diseases. We provide GSL component analysis service by ELISA, and our services include:
The service is suitable for high-throughput automated tests that detect multiple samples simultaneously, with good reproducibility and specificity.
Our services are selective and have a wide range of applications as they provide good results for a wide range of samples. And the process of this service is stable, so the inclusion of serum, tissue, cell products, etc. in the sample does not affect the overall results.
This service allows a relatively low reagent cost, short sample processing time, and stable and reproducible results.
This method of sample processing does not require purification or enrichment and allows quantitative analysis of the raw sample while avoiding the risk of sample loss and compromised accuracy.
Fig.2 ELISA assay at CD BioGlyco. (CD BioGlyco, 2023)
CD BioGlyco has extensive experience in the development and analysis of glycobiology. We have a complete GSL component analysis service process based on ELISA and excellent after-sales service, enabling us to provide high-quality services to our customers. If you are interested in our services, please contact us to obtain more information.
Reference
