Glycosphingolipids (GSLs) are important bio-molecules with diverse and complex chemical structures, and the analysis of their composition is of biological significance. CD BioGlyco offers customized gel electrophoresis (GE) services for GSLs, including starch-gel electrophoresis (SGE), polyacrylamide gel electrophoresis (PAGE), and thin-layer chromatography polyacrylamide gel (TLC-PAG).
GE is a biomolecular separation technology that separates and detects products to be separated depending on their molecular size and charge properties. The basic principle of GE is to use a coagulant to immobilize biomolecules on a gel, and then apply an electrical field to move the biomolecules across the gel for the purpose of separating the products. This technique is used by scientists to separate molecules with different physical properties. GE is commonly used to analyze products, but it can also be used as a pretreatment technique to purify molecules before performing assays such as mass spectrometry, polymerase chain reaction, or DNA sequencing. Different types of GSLs serve different physiological or pathological roles in different cells, organs, and biological systems, so the analysis and research of their components is the thing that many scientists have been doing.
Fig.1 Loading DNA sample into SGE. (Wikipedia)
GSL component analysis is a prerequisite for the study of diseases in which GSLs serve different physiological or pathological roles in various cells, organs, and biological systems. CD BioGlyco has a complete GSL component analysis solution to help clients explore the close relationship between the component of GSLs and disease. Our service includes:
This service provides high separation rates, access to detailed information on GSL components, and good selectivity for GSL, allowing accurate differentiation between GSL and other similar compounds.
This service utilizes fluorescent dye technology during the separation process, allowing visual observation of the separated bands for easy quantification and comparison.
This service combines TLC and PAGE to detect very small amounts of GSL in samples with high separation efficiency and high resolution.
Fig2. The GE technologies at CD BioGlyco. (CD BioGlyco)
CD BioGlyco has accomplished many GSL component analysis projects based on GE with our professional services. Moreover, we adapt our experimental plans to the specific needs of our clients and we constantly update our analytical techniques and solutions to match the needs of our clients. If you are interested in our services, please contact us to obtain more information.
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